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摘要：γ-癸内酯是香料工业应用广泛的一种调香原料。近年来，随着消费者对天然香料的需求与日俱增，生物转化法生产GDL的研究越来越受到各国学者及香料生产企业的重视。微生物发酵获得的产物更为天然、安全，且满足广大消费者的心理需求，已经成为未来天然香料生产的发展趋势。
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本毕业论文主要讨论了几种溶剂乙醇，甲苯， SDS， CTAB，TritonX-100对面包酵母产γ-癸内酯的影响。通过对不同溶剂进行筛选，最终确定了TritonX-100为最佳溶剂，并对其浓度，处理时间，温度以及酵母菌洗涤的次数进行了优化优化，确定了最优处理方法是：TritonX-100浓度为50g／L，处理时间10min，处理温度15℃，洗涤次数2次。在此条件下，面包酵母培养48h后，GDL的发酵产量达到2476.5mg／L。20610
关键词： 有机溶剂；面包酵母；γ-癸内酯；细胞透性
Influence of several organic solvents on the synthesis of γ- decalactone by baker's yeast
Abstract：γ-decalactone is widely used as Perfumery raw materials for industrial applications. In recent years, with the growing consumer demands for natural spices, biotransformation studies gained more and more attentions from various countries and spices manufacturers. The products obtained by microbial fermentation are more natural and safe, and meet the psychological needs of consumers. It has become the development trend of the future production of natural spices.
This paper discussed the influences of several solvents such as ethanol, toluene, SDS, CTAB, TritonX-100 on the γ- decalactone’s producing by baker's yeast. By screening different solvents, the best solvent determined ultimately was TritonX-100, and its concentration, the processing time, temperature and the washing times of the yeast were optimized.The optimal approach was as follows: TritonX-100 concentration was 50g／L, the processing time was 10min, treatment temperature was 10 ℃, washed twice. Under this condition, the yeast was cultured for 48h, and the fermentation yield of GDL reached 2476.5mg／L.
Key words: organic solvents；baker's yeast；γ-decalactone；cell permeability
目 录
1绪论 1
1.1 γ-癸内酯简介 1
1.2 γ-癸内酯生产合成方法 1
1.2.1 天然提取法 1
1.2.2 γ-癸内酯的化学合成方法 2
1.2.3 γ-癸内酯的生物法制取 2
1.3 生物转化法生产γ-癸内酯研究概况 3
1.3.1 国内研究概况 3
1.3.2 国外研究概况 4
1.4 面包酵母概况 5
1.4.1 酵母简介 5
1.4.2 面包酵母作用 5
1.5 丙位癸内酯发酵生产原理 5
1.6 细胞透性 6
1.6.1 物理法 7
1.6.2 化学法 7
1.6.3 生物法 8
1.6.4 分子生物法 8
1.7 透性化酵母细胞国内外研究概况 8
1.8 研究意义 9
2实验材料和方法 10
2.1 材料 10
2.1.1 菌种及培养基配方 10
2.1.2 试剂 10
2.1.3 主要仪器设备 11
2.2 方法 11
2.2.1 酵母培养 11
2.2.2 溶剂处理方法 11
2.2.3 溶剂选择 12
2.2.4 Triton X-100处理方法优化 12
2.2.5 γ-癸内酯的萃取 13