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摘要纳米纤文具有直径小、比表面积大以及易于实现表面功能化的优点，同时由于超细纤文表面的多孔结构，可以制备载药支架，因此更加受到生物医学方面研究人员的重视。本实验将以三氟乙醇作为溶剂，选用聚乳酸 (PLA，200kD)为纺丝纤文基体，以BSA为模型药物，采用不同的喷射速度（如外壳为1.8ml/h,芯为0.6ml/h），通过高压静电纺丝设备，分别制成含不同药物浓度的核壳结构聚乳酸载药纺丝纤文膜。通过扫描电子显微镜观察超细纤文的微观结构，并对超细纤文膜药物释放规律进行了研究，分别考察了不同载药制备工艺和不同纺丝液浓度对蛋白质释放情况的影响。研究发现，所得纺丝纤文微观形貌平直光滑，纺丝直径均一。药物释放结果显示，同轴法得到的载药纤文药物爆释现象明显弱于共混法制得的纤文，且释放缓慢，能够起到很好的缓释作用。
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毕业论文关键词：聚乳酸；BSA；静电纺丝；SEM；药物释放22445
Preparation and drug release properties of the drug-loaded porous drug loaded fibers
Abstract
The advantages of nano fibers are small diameter with large specific surface area and easy to realize the surface functionalization. Owing to the porous structure of superfine fiber surface, they can be used to make drug scaffolds, which greatly draw much more attention by researchers from biomedical field. In this experiment, Trifluoroethanol was used as solvent, polylactic acid (PLA，200kD) as spinning fiber matrix, and BSA as model drug. Different concentrations of PLA drug spinning fiber membranes were made by electrospinningtechnique. The microstructures of superfine fibers were observed through SEM. Besides, the behavior of drug release were studied, including the influences of different preparation procedures and spinning liquid concentrations. To fabricate the smooth and uniform nanofibers, we optimaize the preparation parameters, such as the shell flow rate of 1.8ml/h, core flow rate of 0.6ml/h with the PLA concentration of 12%. The microstructure of the spinning fibers were shown instraight ,smooth in the almost same diameters. The drug release behavior shows that the initial burst release of drug encapsulated by coaxial procedure is significantly decreased, and in a sustained release of BSA after that.
Keywords: polylactic acid (PLA); BSA; electrostatic spinning;SEM; drug release.
目录
1绪论 1
1.1治疗皮肤损伤的天然药物研究进展 1
1.2药物包埋制备工艺的研究进展 2
1.2.1微囊包埋药物的方法 2
1.2.2 静电纺丝包埋药物 4
1.3静电纺丝法的优势 5
1.4药物包埋材料的研究进展 6
1.5本课题研究的目的及意义 7
1.6本课题研究的主要内容 8
2实验方法 9
2.1实验仪器 9
2.2实验原料 9
2.3实验方法 10
2.3.1 PLA纺丝溶液的配置 10
2.3.2牛血清蛋白（BSA）溶液的配置 10
2.3.3考马斯亮蓝G-250的配置 10
2.3.4磷酸盐缓冲液PBS （PH=7.4）的配制 10
2.3.5蛋白质标准曲线的绘制 11
2.3.6静电纺丝薄膜的制备过程 11
2.3.7载药纤文薄膜的微观形貌观察 12
2.3.8 PLA载药纤文膜的亲水性 12
2.3.9药物释放行为的研究 12
2.4结果与讨论 12
2.4.1影响静电纺丝制备的因素 12
2.4.2成功的静电纺丝过程 16
2.4.3 PLA载药纤文膜的亲水性研究 17