摘要纳米金刚石作为新兴的纳米材料在各个领域都有所展露，在生物医药方面更是有了许多新的进展。纳米金刚石有着极好的生物相容性，无毒性，能非常有效的提高药物的生物利用度。本次设计首先利用密度梯度超速离心法筛选出粒径均匀且小于3nm的纳米金刚石。纳米金刚石载带水溶性药物和非水溶性药物的不同。通过纳米金刚石和阿霉素的多种搭建方式的计算得出纳米金刚石载带多柔比星的最佳理论载药量。用酰胺化反应将纳米金刚石与PEG或者主动靶向分子如叶酸等化学结合来达成免疫隐身修饰。对纳米金刚石的聚集问题进行粒径的调控，通过小分子DNA或者脂质体调控非水溶性药物的粒径，以及通过PEG的修饰来调控水溶性药物的粒径。使得纳米金刚石载带的药物能够在人体中发挥更好的疗效。80602

毕业论文关键词：纳米金刚石载药系统 粒径调控 隐身修饰 理论载药量

Abstract Nano-diamond as emerging nano-materials have been exposed in various fields, in the biomedical side is a lot of new progress。 Nano-diamond has excellent biocompatibility, non-toxic, can effectively improve the bioavailability of drugs。 In this design, nano-diamond with uniform particle size and less than 3nm was screened by density gradient ultracentrifugation。 Nano-diamond loaded with water-soluble drugs and non-water-soluble drugs are different。 The optimum theoretical loading of doxorubicin loaded with nano-diamond was calculated by the method of nano-diamond and adriamycin。 Amide reaction with nano-diamond and PEG or active target molecules such as folic acid and other chemical combination to achieve immune stealth modification。 The particle size of the water-soluble drug is regulated by the particle size of the nano-diamond aggregation, the particle size of the water-insoluble drug is regulated by the small molecule DNA or the liposome, and the particle size of the water-soluble drug is regulated by PEG modification。 So that nano-diamond carrier drugs can play a better effect in the human body。

Key words:  nano - diamond drug - loaded system    particle size control   stealth modification   theory drug loading

目录

1。 绪论 6

1。1 设计背景 6

1。2 国内外研究现状 7

1。3本次设计的基本内容和方法 7

2。对纳米金刚石前期处理 8

2。1 纳米金刚石的性质和用途 8

2。2 纳米金刚石的药物传递 8

2。3 纳米金刚石的纯化和粒径筛选 9

2。4 阿霉素与5-FU对纳米金刚石粒径的影响 10

3。纳米金刚石载药量的理论计算 11

3。1 纳米金刚石的模型构建 11

3。2阿霉素在六边形平面上水平搭建（图2。34） 12

3。2。1由以对角线平行搭建阿霉素 13

3。2。2以六边形对边中点连线平行搭建阿霉素 14

3。3 阿霉素在六边形平面上水平搭建 14

3。4 纳米金刚石理论载药量 15

3。5竖直搭建 15

3。6 竖直平躺混合搭建 15

4。对纳米金刚石载药系统粒径的调控 15