摘要：咖啡酸苯乙酯(CAPE) 作为蜂胶中的特征性之一，具有抗癌、抗病毒等多种药理活性，但由于水溶性差必将影响其在体内的吸收。本论文通过将CAPE负载在纳米碳酸钙微球上，提高其溶解度。咖啡酸作为晶型调控剂，室温制备vaterite型纳米碳酸钙。利用XRD、SEM、BET、粒度仪等检测手段对不同条件下合成的载药材料进行形状表征，寻找最优的合成条件制备比表面积大、团聚少、尺寸均一、形貌好的vaterite型碳酸钙。对CAPE进行吸附条件的考察，并在不同pH值的磷酸缓冲液中进行CAPE@CaCO3的释放研究，在48小时内，累计释放率均达到了25%，提示该复合微球具有缓释的作用。72163

毕业论文关键词： CAPE；碳酸钙；纳米微球；载药

Preparation and Characterization of CAPE Nanoparticles

Abstract: Caffeic acid phenate (CAPE) as one of the characteristics of propolis, with anti-cancer, anti-virus and other pharmacological activity, but because of poor water solubility will affect its absorption in the body。 In this paper, CAPE was loaded on nano-calcium carbonate microspheres to improve its solubility。 Caffeic acid as a crystal modifier, and the calcium chloride and sodium carbonate solution were mixed and stirred at room temperature to prepare vaterite type nano calcium carbonate。 The morphology of the drug - loaded materials synthesized under different conditions was characterized by XRD, SEM, BET, particle size analyzer and so on。 The optimum conditions were obtained。 The vaterite type calcium carbonate with different surface area, small agglomeration, uniform size and good appearance was prepared。 The CAPA CaCO3 release was studied in the phosphate buffer with different pH value。 The cumulative release rate reached 30% within 48 hours, which indicated that the composite microspheres had sustained release effect。

Key words:  CAPE;   calcium carbonate;   nanospheres;   drug loading

目录

1．文献综述 1

1。1研究背景 1

1。2碳酸钙纳米微球材料 1

1。3纳米微球晶型的控制 1

1。3。1酸类晶型控制剂 1

1。3。2无机盐类晶型控制剂 2

1。3。3醇类晶型控制剂 2

1。3。4氨基酸晶型控制剂 2

1。4 CAPE相关的研究概况 2

1。4。1 CAPE的药理作用 4

1。5碳酸钙的测定方法及其负载CAPE能力的测定方法 5

1。5。1 XRD 5

1。5。2 SEM 5

1。5。3 BET 5

1。5。4 HPLC 5

1。6 选题理由 6

2．CAPE纳米微球的制备及选型方法 6

2。1 实验所需药品及仪器 6

2。2 实验步骤及纳米微球表征结果 7

2。2。1控制不同CA浓度制备碳酸钙微球 7

2。2。2不同CA下浓度纳米微球表征 8

2。2。3 4mg/ml CA浓度下不同反应时间制备纳米微球 11

2。2。4 4mg/ml CA浓度下不同反应时间制备纳米微球的表征 12

3。 CAPE的吸附与解吸附实验