摘要毛管电色谱作为一种新型的微分离分析技术，结合了毛细管电泳的高效性和高效液相色谱高选择性的优点，同时因为其兼有高分离效率和较小化学药品消耗量而具有广泛而重要的应用潜力。毛细管填充柱、开管柱和整体柱是毛细管电色谱中常用的3种柱制备类型，毛细管整体柱由于易于制备、具有较好的机械加工性能和通透性，以及固定相功能基团易于修饰而备受关注。氧化石墨烯不仅具有强的π-π电子堆集效应和大的比表面积，而且提供了活性的含氧官能团，便于修饰到整体柱固定相表面，同时对样品具有一定的富集作用。环糊精是一类广普性的手性识别试剂，对许多药物具有手性识别功能。88176

本论文最终目的是制备含有环糊精－氧化石墨烯功能基团的聚苯乙烯毛细管电色谱整体柱。目前开展的主要工作是探讨未修饰环糊精时纯聚苯乙烯和含氧化石墨烯功能基团的聚苯乙烯毛细管电色谱整体柱制备条件的优化。

Capillary electrochromatography, as a novel micro-separation analysis technique, combines the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography, and has a wide range of high efficiency due to its high separation efficiency and small chemical consumption Important application potential。 The capillary packed column, the open column and the monolithic column are the three types of column preparation commonly used in capillary electrochromatography。 The capillary monolithic column is easy to prepare, has good machinability and permeability, and the stationary phase functional group is easy to modify And concern。 The graphene oxide not only has strong π-π electron stacking effect and large specific surface area, but also provides active oxygen-containing functional groups, which can be easily modified to the surface of the whole column stationary phase, and have some enrichment effect on the sample。 Cyclodextrin is a broadly universal chiral recognition reagent that has a chiral recognition function for many drugs。

The final aim of this paper is to prepare monolithic polystyrene capillary electrochromatography columns containing cyclodextrin-fossilene functional groups。 The main work of this paper is to optimize the preparation conditions of polystyrene capillary electrochromatography monolithic column with alkene-free functional groups of unmodified cyclodextrin。

毕业论文关键词：氧化石墨烯；环糊精；电色谱；整体柱；制备

Keyword: Graphene oxide；Cyclodextrin；electrochromatography；monolithic columns；preparation

目    录

1  引言 5

1。1毛细管电色谱源-于,优W尔Y论L文.网wwW.youeRw.com 原文+QQ75201,8766简介 5

1。2 石墨烯和环糊精简介 5

1。3石墨烯、氧化石墨烯及应用 6

1。4环糊精及其应用 7

2  石墨烯-环糊精毛细管电色谱整体柱的制备 9

2。1 实验部分 9

2。1。1 实验试剂 9

2。1。2 实验仪器 9

2。1。3 毛细管预处理 9

2。1。4 氧化石墨烯手性整体柱的制备 10

2。2 聚苯乙烯整体柱制备条件的优化 11

2。2。1 交联剂二乙烯苯对整体柱的影响 11

2。2。2单体苯乙烯对整体柱的影响