摘   要目的：大量研究表明野生桑黄(Inonotus SangHuang)水提物具有抗肝纤维化特性。然而对于仿生桑黄的药理作用研究较少，因此本实验选用仿生桑黄作为实验对象研究仿生桑黄水提物抗肝纤维化的特性。以明确仿生桑黄对野生桑黄在肝病治疗上的替代意义。80803

方法：实验用四氯化碳（CCL4）诱导小鼠肝纤维化模型，以仿生桑黄J为原料，探究仿生桑黄水提物对改善四氯化碳诱导小鼠肝纤维化的影响，将80只ICR雄性小鼠随机分成空白对照组、模型组、桑黄组、护肝片组四组，每组20只。空白对照组腹腔注射100%花生油1 ml/kg，每周3次，共十周；模型组腹腔注射20%四氯化碳的花生油1 ml/kg，每周3次，共十周；桑黄组腹腔注射20%四氯化碳的花生油1 ml/kg，每周3次，共十周。并从第五周起给予仿生桑黄多糖10 mg/（kg。d）灌胃至第十周；护肝片组腹腔注射20%四氯化碳的花生油1 ml/kg，每周3次，共十周。并从第五周给予护肝片1 g/（kg。d）灌胃至第十周。第十周给药结束后分析小鼠肝脏指数、血清指标、小鼠增重、肝脏形态学特征和肝脏病理组织切片。

结果：模型组小鼠血清指标和肝脏指数较空白对照组小鼠有明显的差别（P<0。05），桑黄组和护肝片组血清指标和肝脏指数较模型组有所改善。病理学组织切片显示模型组出现肝细胞高度水肿变性、炎症细胞、坏死、再生性结节和纤维组织增生。桑黄组和护肝片组小鼠肝细胞轻度水肿变性，并未出现脂肪变，轻微发炎。模型组小鼠体重较空白对照组增长缓慢甚至出现逆生长，桑黄组和护肝片组治疗之前体重增长缓慢，治疗之后体重增长逐渐正常。模型组肝脏形态学特征较空白对照组差异明显，表现在形状变形、粗糙、暗淡。桑黄组和护肝片组肝脏形态学特征较空白对照组差异不明显。本项研究证明仿生桑黄水提物对四氯化碳诱导肝纤维化小鼠具有改善作用，为今后广泛的开发利用仿生桑黄代替野生桑黄作为治疗肝病的新药物提供了理论依据。

毕业论文关键词：仿生桑黄水提物  肝纤维化  谷丙转氨酶  谷草转氨酶

Abstract Objective: the study shows that a large number of wild Phellinus linteus (Inonotus SangHuang) water extract has anti fibrotic properties。 However for less research on pharmacological action of bionic Phellinus igniarius, so the selection of bionic Phellinus igniarius as experimental object on the biomimetic Mulberry Yellow Water Extract of anti hepatic fibrosis。 In order to clear the significance of alternative bionic Phellinus linteus on wild Phellinus in the treatment of liver diseases。

Method: the experimental carbon tetrachloride (CCl4) - induced liver fibrosis model in mice, with bionic J of Phellinus igniarius as raw material, research on bionic mulberry yellow water extract to improve the carbon tetrachloride induced liver fibrosis in mice, 80 male ICR mice were randomly pided into blank control group, model group, Huang sang group, Hugan tablets group four groups, 20 rats in each group。 Blank control group received intraperitoneal injection of 100% peanut oil 1ml / kg, 3 times per week, for a total of ten weeks; model group, the intraperitoneal injection of 20% CCl4 Peanut Oil 1ml / kg, 3 times per week, for a total of ten weeks; Phellinus linteus group were intraperitoneally injected with 20% CCl4 Peanut Oil 1ml / kg, 3 times per week, a total of ten weeks。 And from the fifth week given bionic Phellinus polysaccharide 10mg / (kg-d) fill the stomach to the tenth week; Hugan tablet group were intraperitoneally injected with 20% CCl4 Peanut Oil 1ml / kg, 3 times per week, a total of ten weeks。 And give huganpian (kg。d) 1g/ from fifth weeks to tenth weeks by gavage。 After tenth weeks administration, the indexes of serum, liver index, pathological histology section, mice weight gain, liver morphology were analyzed。