摘要桑黄是一种寄生于腐烂桑树上的多年生真菌。大量科学研究发现桑黄具有调节免 疫功能，从而达到抑制肿瘤细胞生长的作用，其抗癌率高达 96。7%，是目前世界上公 认的抗肿瘤效果最佳的真菌。为探索仿生桑黄水提物的体外抗肿瘤活性，本实验对比 研究了仿生与野生桑黄体外抗肿瘤效果，实验千岛湖野生桑黄 O 和仿生桑黄 J 为原 材料，对比研究了野生桑黄和仿生桑黄水提物对小鼠肾上腺嗜铬细胞 PC12 及肝癌细 胞 HGC、胰腺癌细胞株 Patu8988、胰腺癌细胞株 SW1990 和胃癌细胞 A549 这四种 肿瘤细胞生长的影响。结果表明：野生桑黄 O 和仿生桑黄 J 水提物中的多糖对各肿 瘤细胞的生长均有一定的抑制作用，其中对肿瘤细胞 1990 的抑制效果最明显，仿生 桑黄抑制率高达 70%，野生桑黄抑制率为 40%。并且在一定浓度范围内，抑制率随 溶液浓度增加而提高。但是对正常小鼠肾上腺嗜铬 PC12 的生长基本无影响，细胞形 态未发生显著改变，两种桑黄的抑制率均接近于 0。本研究表明桑黄水提物能够有效 抑制癌细胞的增殖而对正常的细胞基本无影响，且仿生桑黄对肿瘤细胞的抑制效果优 于野生桑黄，仿生桑黄可以替代野生桑黄。抑制效果与桑黄多糖浓度呈现出显著的正 相关的趋势，为桑黄的进一步开发利用提供了理论依据。80729

毕业论文关键词：桑黄；多糖；免疫活性；抗肿瘤

Abstract Sang Huang is a perennial and perennial fungi on putrid mulberry。 A large number of scientific research prove that Sang Huang has immunoregulatory function, thereby it can inhibiting tumor cell grow。 Be independent of best antineoplastic Fungi in the world, Sang Huang reach 96。7 rata of cells inhibition。 In order to explore the antitumor activity in vitro of aqueous extract of bionic Inonotus Sang Huang, regarding Inonotus Sang Huang of Qingdao Lake O and bionic Inonotus Sang Huang J as raw material, contrast the effect of the water extract of the wild Inonotus Sang Huang and the bionic Inonotus Sang Huang on the growth of    mouse adrenal pheochromocytoma cell PC12 and some cancer cells like

hepatoma carcinoma cell HGC、pancreatic cancer cell Patu8988、pancreatic cancer    cell

1990 and Gastric cancer cell A549。The results show that both the wild Inonotus Sang Huang O and the bionic Inonotus Sang Huang J can restrain cancer cells grow but have no effect on normal mice cells。 By comparison experiments, it’s turned out that the both sides of the two Sang Huang have anti-tumor effects to all cancer cells。 Among them, the inhibitory effect on tumor cell 1990 was the most obvious。 The inhibition rate of bionic Inonotus Sang Huang as high as 70,and the inhibition rate of wild Inonotus Sang Huang   is

40。 In a certain concentration range, the inhibition rate increases with the increase of the solution concentration。 But it has no effect on the growth of the mouse adrenal pheochromocytoma cell PC12。The antitumor experiment in vitro shows that the both sides have the anti-tumor effects but they Have no effect on normal cells。。 The bionic Inonotus Sang Huang J better than the wild Inonotus Sang Huang O on inhibiting tumor cell grow。 So the bionic Inonotus Sang Huang can replace the wild Inonotus Sang Huang 。And it exhibit significantly that the aqueous extract concentration was positively correlated with the inhibitory effect of the trend, which provides an important theoretical basis for utilization and further development of Inonotus Sang Huang。

Keywords: Inonotus Sang Huang；polysaccharide；Immune activity；anti-tumor

目 录

第一章 绪论 1

1。1 引言 1

1。1。1 桑黄简介