新型可注射水凝胶软骨再生支架的制备

摘要本研究在引发剂Irgacure 2959的作用下，以甲基丙烯酸酯化的水溶性壳聚糖（N-琥珀酰壳聚糖，SCS）和聚乙二醇（PEG）为基体，通过紫外光引发聚合交联，得到了复合水凝胶材料。在37oC磷酸盐缓冲盐水（PBS）中，对加入PEG后的SCS/PEG复合水凝胶体外形态、平衡溶胀和失重进行了研究。为了评价水凝胶是否能有效传递脂肪细胞因子能力，实验中胰岛素被包埋在水凝胶共聚甲基丙烯酸酯化的SCS/PEG中。我们发现胰岛素能通过控制自身内在SCS/PEG重量比的释放来调节，从而融入到凝胶所处的环境。结果表明，添加重量比为10%PEG的复合水凝胶负载较多的胰岛素，表现出较高的包埋效率，有望用于脂肪等软组织工程。68054

毕业论文关键词：聚（乙二醇），壳聚糖，水凝胶，胰岛素，组织工程

毕业设计说明书（论文）外文摘要

Title The Preparation of Novel Injectable Hydrogel Scaffolds for Cartilage Regeneration

Abstract

Biodegradable hydrogels carrying adipose-derived stem cells (ASCs) have been highlighted with promising potential regarding adipose tissue engineering. In this study, covalently crosslinked natural/synthetic hybrid hydrogels were prepared from methacrylated water soluble chitosan (N-succinyl-chitosan, SCS) and poly(ethyleneglycol) (PEG) by photoinitiaing polymerization under the existence of Irgacure 2959 and the irradiation of UV light. The effect of the incorporated PEG on the in vitro morphologies, equilibrium swelling, weight loss and compressive modulus of SCS/PEG hybrid hydrogels in phosphate buffered saline (PBS) at 37oC were studied. In order to evaluate the ability of hydrogels to effectively deliver the adipogenic factor, insulin was entrapped within hydrogels by copolymerizing methacrylated SCS/PEG. The insulin would be released from the hydrogels into the local microenvironment via the controlled weight ratio of SCS/PEG. Results demonstrated that the hybrid hydrogel with 10 wt% PEG showed a higher efficiency of insulin delivery compared to the control hydrogels. ASCs were seeded into the insulin-loaded SCS/PEG hydrogels in vitro to assess the biological performance and applicability of hydrogels as cell carriers. These characteristics provide potential opportunities for the hybrid SCS/PEG hydrogels as injectable scaffolds in soft tissue engineering applications.

Keywords：poly(ethylene glycol), chitosan, biodegradable hydrogel, insulin, adipose tissue engineering

1 前言 1

1．1 研究背景 1

1.1.1组织工程的介绍 1

1.1.2用于组织工程的生物材料 2

1.1.3壳聚糖作为生物材料的应用情况 2

1.2课题的提出 4

2 实验部分 6

2.1 材料 6

2.2 琥珀酰壳聚糖（SCS）的合成 6

2.3 甲基丙烯酸酯化壳聚糖的合成 6

2.4 聚乙二醇二甲基丙烯酸酯的合成 6

2.5 SCS/ PEG水凝胶的制备 6

2.6 微观形态 7

2.7 平衡溶胀 7

2.8 重量损失 7

2.9 胰岛素的包埋 7

3 结果与讨论新型可注射水凝胶软骨再生支架的制备:http://www.youerw.com/cailiao/lunwen_76466.html