多糖合成途径中功能基因的鉴定及其在粘细菌发育中的研究(4)
时间:2018-07-10 10:37 来源:毕业论文 作者:毕业论文 点击:次
pMD19-T- 5333 pMD19-T derivative carrying MXAN_5333 This study pMD19-T- 6908 pMD19-T derivative carrying MXAN_6908 This study pBJ113 Derived from PUC118 with galK and Kanr Yang Zhaoming and Mao Xiaohua pBJ113-2921 pBJ113 derivative carrying MXAN_2921 This study pBJ113-3506 pBJ113 derivative carrying MXAN_3506 This study pBJ113-4613 pBJ113 derivative carrying MXAN_4613 This study pBJ113-5333 pBJ113 derivative carrying MXAN_5333 This study pBJ113-6908 pBJ113 derivative carrying MXAN_6908 This study 1．2 引物 表2-1-2实验所用引物 Table 2-1-2 Oligonucleotide primers used in the experiment 引物 序列 (5'-3') 设计目的 2921F CCCAAGCTTGTCCTTGATGCCCTCTGCG Forward primer for MXAN_2921 homologous fragment 2921R CCGGAATTCAAGCCTGCTTCCCATTCGTC Reverse primer for MXAN_2921 homologous fragment 3506F CCCAAGCTTGAAGCGGGTAGCGGTCCT Forward primer for MXAN_3506 homologous fragment 3506R CCGGAATTCGGAACCACGCAATGGTCTC Reverse primer for MXAN_3506 homologous fragment 4613F CCCAAGCTTGGTGGCTGTGGCTTCATCG Forward primer for MXAN_4613 homologous fragment 4613R CCGGAATTCCGCGTTCGGTACTGCGTTTC Reverse primer for MXAN_4613 homologous fragment 5333F CCCAAGCTTGGCATCGTCTTCCACAACATCA Forward primer for MXAN_5333 homologous fragment 5333R CCGGAATTCTTCACGCAGCCGCTCGTT Reverse primer for MXAN_5333 homologous fragment 6908F CCCAAGCTTCCGAGCGAGAACTGTGGGAG Forward primer for MXAN_6908 homologous fragment 6908R CCGGAATTCTGGAGCCCTTGTTGCCGTA Reverse primer for MXAN_6908 homologous fragment 2921tF TGCCCTGGTCCACGATTG Forward primer for identifying △MXAN_2921 2921tR TCTGGCAACACCTCCCCC Reverse primer for identifying △MXAN_2921 3506tF ACGTTCGACGAGCTGTGAGAG Forward primer for identifying △MXAN_3506 3506tR GACCTTCACGCAACCTCCC Reverse primer for identifying △MXAN_3506 4613tF TGGAGCAAGGAACACACGC Forward primer for identifying △MXAN_4613 4613tR GTGGACCCCTTCGATACGC Reverse primer for identifying △MXAN_4613 5333tF CCCGGTGACCTGGTCCTCT Forward primer for identifying △MXAN_5333 5333tR TGGGCGCGTAGAGGTAGGT Reverse primer for identifying △MXAN_5333 6908tF GCGAAAACTGCCAGGAGGA Forward primer for identifying △MXAN_6908 6908tR CGATGAAGGTGGCGAAGAA Reverse primer for identifying △MXAN_6908 2921exF TCCCCGGCATCCACGCGCGCTA Forward primer for testing the expression of MXAN_2921 2921exR GTTCCGAAGCCTGGATGCGCA Reverse primer for testing the expression of MXAN_2921 3506exF GGCCTCGCCGGCCTCGCCCA Forward primer for testing the expression of MXAN_ 3506 3506exR GTCTACGACGAGGCCAAGCG Reverse primer for testing the expression of MXAN_3506 4613exF CGCCGGCCCGGCCCTTCTCCA Forward primer for testing the expression of MXAN_4613 (责任编辑：qin) |