邻苯二甲酸二甲酯和邻苯二甲酸二乙酯对三角褐指藻的毒性效应
试验通过对三角褐指藻的培养，接种培养后，将邻苯二甲酸二甲酯和邻苯二甲酸二乙酯设置成0、50、100、200、400 mg/L，以及将邻苯二甲酸二甲酯与邻苯二甲酸二乙酯按200：0、200：50、200：100、200：200、200：400 mg/L的浓度梯度进行毒性试验，设置三个平行组，每隔24 h进行OD625、OD645、OD663的数据采集；持续96 h后，进行细胞破碎，利用试剂盒测量SOD、POD、MDA以及总蛋白含量。通过与对照组比较，实验结果表明：邻苯二甲酸二甲酯二甲酯在50、100、200、400 mg/L的抑制率分别为：9。5%、22。7%、35。2%和72。7%；邻苯二甲酸二乙酯在50、100、200、400 mg/L浓度是的抑制率分别为：25。4%、80。5%、103。4%、121。3%；所设置按比例耦合浓度的抑对藻体的抑制率分别为：54。5%、80。3%、103。2%、126。1%。得出结论：随着邻苯二甲酸二甲酯和邻苯二甲酸二乙酯的浓度不断升高，对藻体的毒性作用越来越大，邻苯二甲酸二乙酯比邻苯二甲酸二甲酯对三角褐指藻的毒性更大，两者耦合后的抑制能力更强。通过分析破碎细胞后对三角褐指藻各项指标的测量结果，发现：邻苯二甲酸酯类影响三角褐指藻的正常生命活动，随着浓度增高，藻体内氧自由基不断增多，细胞破损情况越来越严重，邻苯二甲酸酯类物质浓度过高时，藻体会直接死亡。
毕业论文关键字：邻苯二甲酸二甲酯；邻苯二甲酸二乙酯；三角褐指藻；毒性作用
Abstract PAEs are the most widely and usely plasticizers in human life。 PAEs have genotoxicity and water can be polluted by PAEs which is endocrine disrupters。 It can make animals with adverse impacts such as teratogenicity, carcinogenic and mutagenesis。 Phacodactylum tricornutum is one kind of marine unicellular algae; and it is theprimary producer which at bottom of the food chain。 The phacodactylum tricornutum is smaller inpidual, which is beneficaial to cultivate and research。 This experiment is aim to measure the toxicity effect of DMP and DEP to Phacodactylum tricornutum。
We will inoculate and cultivate Phacodactylum tricornutum in this experiment, and set the concentration of DMP and DEP as 0, 50, 100, 200, 400 mg/L, then couple the DMP and DEP to interfere the Phacodactylum tricornutum by the concentration of 200:0, 200:50, 200:100, 200:200, 200:400 mg/L。 We seted up three parallel groups, then collect data of OD625, OD645, OD662 each 24 hours within 96 hours。 we broken the cells,measured the SOD, POD, MDA and total protein。 By comparing with control group, the test result show that the inhibition ratio of DMP to deal the Phacodactylum tricornutum in the concentration of 0, 50, 100, 200, 400 mg/L is 9。5%, 22。7%, 35。2% and 72。7%; the inhibition ratio of DEP to deal the Phacodactylum tricornutum in the concentration of 0, 50, 100, 200, 400 mg/L is 25。4%, 80。5%, 103。4% and 121。3%; the inhibition ratio of coupling DEP and DEP to deal the Phacodactylum tricornutum is 54。5%, 80。3%, 103。2% and 126。1%。 We reach the conclusion that with the growth of DMP and DEP’ concentration, the toxicity to Phacodactylum tricornutum is more and more strong, the toxicity of DMP to the Phacodactylum tricornutum is biger than DEP’s。 The toxicity of coupling is stronger than DMP or DEP。 By analyzing the NPQ, QY, SOD, POD, MDA and total protein of phacodactylum tricornutum, we can find that PAEs can restrain the algae’s biogenic activities。 With the growth of DMP and DEP’s concentration, the number of oxygen free radical of phaeodactylum is increase, broken cell rate go from bad to worse。 When the concentration is over the level, phaeodactylum is got to die immediately。