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摘要：洛神花的价值已在食品、药品等多个方面得到验证，具有很好的应用前景。本文主要采用35%、45%、55%、65%、75%、95%浓度乙醇作为溶剂时, 研究了洛神花有效成分的提取工艺，并采用酪氨酸酶对粗提物的美白功效进行评价，并在此基础上进一步提取了粗提物中的多糖成分，对多糖的保湿性和在基质膏霜中的应用等进行了研究。结果表明：1）洛神花提取物具有一定的美白功效，且使用75%乙醇提取的洛神花粗提物的酪氨酸酶抑制率最佳。2）将多糖粗提物进行失水和吸湿的实验，发现洛神花具有较好的保湿性能，在相对湿度为81%时，失水率为60%，吸水率为20%-30%，在相对湿度为43%时，失水率为20%，吸水率为70%-120%，与甘油相比，吸湿性略差但有更好的锁水能力。多糖在基质膏霜中有较好的配伍性，耐热耐寒稳定性良好，无分层或者析出现象。22513
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可见洛神花在化妆品美白以及保湿方面有一定的应用前景，可作为植物提取物加入化妆品中。
毕业论文关键词： 洛神花；紫外分光光度法；多糖；保湿；美白
Extract the effective components of Roselle and evaluation whitening effect
Abstract: The value of Roselle has been validated in many aspects such as food, medicine, etc., with good prospects. In this paper, we use 35%,45%,55%,65%,75%, 95% concentration of ethanol as a solvent, discussing the extraction process of Roselle, using tyrosinase measuring the whitening effect on the crude extract of Roselle. And on this basis, we extract the crude extract polysaccharide components and applications of moisturizing creams in the matrix of polysaccharides, etc. are studied. The results show that: 1) Roselle extract has the whitening effect, and 75% ethanol extract of crude extract of Roselle has the best tyrosinaseinhibition rate. 2) Roselle crude extract of polysaccharide shows the good results in water absorption and water loss test. At a relative humidity of 81%, water losing rate is 60%，water absorption rate is 20%-30%. At a relative humidity of 43%, water losing rate is 20%，water absorption rate is 70%-120%. Compared with glycerol, its water absorption is poor, but its water locking ability is better. It is proved that Roselle polysaccharide crude extract added to emulsion is stable. Sample is no stratified or separation.
It also shows Roselle has a good prospect of application on whitening、moisturizing as a planting extraction in cosmetics production.
Key word: Roselle; UV spectrophotometry; Polysaccharide; Moisture; Whitening
目录
1.前言 1
1.1 化妆品发展现状 1
1.2.洛神花国内外研究现状 1
1.2.1洛神花有效成分 2
1.2.2 洛神花有效成分的应用 3
1.3本课题研究的目的和意义 3
2.实验原理与方法 5
2.1 实验原料与仪器设备 5
2.1.1 实验原料 5
2.2 实验步骤 7
2.2.1 洛神花粗提物的提取 7
2.2.2洛神花粗提物美白效果的测定 10
2.2.3洛神花中多糖的提取 11
2.2.4洛神花中多糖保湿性的测定 12
2.2.5 膏霜的配制 12
2.2.6含洛神花成分膏霜性能的测试方法 14
3.实验结果与分析 15
3.1 不同浓度乙醇对提取率的影响 15
3.2 美白效果的功效测定与分析 15
3.3 洛神花多糖的测定 17
3.4 多糖吸水性的测定 18
3.5 多糖失水性的测定 21
3.6 基质膏霜的配方确定 23