摘要：采用响应面法优化金银花水溶性多糖的酶解法提取工艺，为了提高金银花水溶性多糖的得率，对纤维素酶的酶质量分数、酶解时间、酶解温度、酶解pH值四个要素分别进行单因素试验，再根据单因素实验结果设计响应面实验，以金银花水溶性多糖的提取率为因变量，经过响应面法分析后，确定4个因素的最优工艺参数分别为：纤维素酶质量分数2。2%，酶解时间1。7 h，酶解温度56℃，酶解pH为6，此时水溶性多糖提取率为4。91%，与模型的预测值4。94％非常接近，说明响应面法优化金银花水溶性多糖提取工艺获得成功。80998

毕业论文关键词： 金银花；水溶性多糖；提取；响应面优化

Study on Extraction of Polysaccharides from  Lonicera japonica

Abstract: In order to improve the yield of water-soluble polysaccharides from Lonicera japonica, single factor tests were carried out on the four factors: enzyme mass fraction of cellusale, enzymolysis time, enzymolysis temperature and pH value。 After which the response surface methodology was designed, and the optimum technological parameters of the four factors were determined after the extraction rate of water-soluble polysaccharides of Lonicera japonica was chosen as the dependent factor。 Results showed the optimum process parameters were as follows: enzymolysis time 1。7 h, enzymolysis temperature 56℃, enzymolysis pH 6, and the cellulose enzyme content 2。2%。 Under these conditions, the extraction rate of the water-soluble polysaccharides was 4。91%, which is very close to the expected value 4。94%。 It suggested the optimized extraction technology of water-soluble polysaccharides from Lonicera japonica could be successfully achieved by the response surface methodology。

Key Words: Lonicera japonica; Water-soluble Polysaccharide;Extraction; Response Surface Methodology

目录

1前言 5

1。1植物多糖 5

1。1。1 植物多糖的定义 5

1。1。2 植物多糖的分类 5

1。1。3 植物多糖的应用前景 6

1。1。4 植物多糖的提取方法 7

1。2金银花的简介 8

1。3 响应面优化分析研究 9

1。3。1 定义 9

1。3。2 响应面优化法的优点 10

1。3。3 响应面优化法的不足 10

1。4 研究目的与定义 10

2材料与仪器 10

2。1试验材料与试剂 10

2。2试验仪器 11

2。3实验方法 11

2。3。1工艺流程图 11

2。3。2 操作要点 12

2。4 单因素实验设计 13

2。4。1 单因素实验-酶解时间 13

2。4。2 单因素实验-酶解温度 14

2。4。3 单因素实验-纤维素酶的质量分数 14

2。4。4 单因素实验-酶解pH值 15

2。5响应面分析实验 15

3 结果分析与讨论 17

3。1 单因素试验测定结果