摘要：甘蔗渣是一种常见的固体废弃物，含多种营养成分，然而目前尚无有效地利用手段。本研究以Yarrowia lipolytica SWJ-1b为发酵菌株，以甘蔗渣为唯一碳源，进行赤藓醇的发酵生产。首先通过初步实验结果证明了实验的可行性。为了进一步提高赤藓醇的产量，我们又对发酵条件进行了优化。最终赤藓醇发酵优化结果为：甘蔗渣最佳添加浓度为50。0 g/L；最佳氮源为(NH4)2S2O8且其最佳添加浓度为3。0 g/L；MgSO4·7H2O与KH2PO4的最佳浓度都是1。0 g/L；添加60。0 g/L NaCl所产生的渗透压最有利于赤藓醇生成；同时在培养基中添加10 mg/L的Mn2+ 和4 mg/L的Cu2+ 进一步提高了赤藓醇的产量。在优化培养基中培养144小时，赤藓醇的产量达到最大，为32。6 g/L，产率达到65。2%。该结果表明甘蔗渣可以作为唯一碳源生产赤藓醇，该方法可作为一种有效利用甘蔗渣的新途径，同时探究了一条低成本发酵赤藓醇的新工艺。94651

毕业论文关键词：赤藓醇，解脂耶氏酵母，甘蔗渣，发酵优化

Abstract: Cassava bagasse is nutrient-rich agro-industrial residues which have the potential to be used as substrates for erythritol production, whereas it has not been effectively used。 In this study, Yarrowia lipolytica SWJ-1b was used as the fermentation strain, and the bagasse was the sole carbon source。 The fermentation of erythritol was carried out。 The feasibility of the experiment is proved by preliminary experimental results。 In order to improve the yield of erythritol, we also optimized the fermentation conditions。 As a result, it was found that the optimum concentration of bagasse was 50g/L。 (NH4)2S2O8 was the best nitrogen source and its optimum concentration was 3。0 g/L。 The optimum concentration of MgSO4·7H2O and KH2PO4 was 1。0 g/L。 And the osmotic pressure of 60。0 g/L of NaCl was the most favorable for the formation of erythritol。 At the same time, adding 10 mg/L of Mn2+ and 4 mg/L of Cu2+ in the medium further improved the production of erythritol。 The yield of erythritol was the highest at 32。6 g/L and the yield reached 65。2% in 144 h in the medium。 The results show that bagasse can be used as a sole carbon source to produce erythritol, which can be used as one of the ways to reuse sugarcane bagasse and a new process of low cost erythritol fermention is also explored。

Key words: Erythritol, Yarrowia lipolytica, Bagasse, Fermentation optimization

目   录

1  前言 4

2  材料和方法 8

2。1  材料 8

2。2  方法 9

3  实验结果与讨论 10

3。1  原料的预处理 10

3。2  标准曲线的绘制 10

3。3  碳源浓度对Y。 lipolytica赤藓醇发酵的影响 10

3。4  NaCl浓度对赤藓醇发酵的影响 12

3。5  氮源种类对Y。 lipolytica赤藓醇发酵的影响 13

3。6  氮源浓度对Y。 lipolytica赤藓醇发酵的影响 14

3。7  添加Cu2+和Mn2+对赤藓醇发酵的影响 15

3。8  赤藓醇发酵过程细胞内代谢流分析 16

3。9  Y。 lipolytica发酵产赤藓醇曲线的测绘 17

结  论 18

参 考 文 献