摘要微生物燃料电池的启动、运行过程中，反应器所处运行环境将影响电池的性能。本研究旨在考察电池启动期的运行特征，及电池启动期阳极、阴极环境因子对电池反应器系统性能的影响，为后续运行提供运行指标参考。63854

本课题采用分批方式运行双室微生物燃料电池，考察了电池启动期电压输出，及pH、温度、电导率、ORP等环境因子的变化规律，使用SPSS统计分析软件进行了产电与环境因子的相关性分析，并对具硝基酚还原功能的生物阴极启动情况进行了研究。

实验结果表明，双室电池启动过程中，输出电压与阳极电导率、阴极ORP呈显著相关，与阳极、阴极pH均呈极显著相关，后续稳定运行中pH应为物化条件调控的重点。生物阴极启动过程中，可实现硝基酚的降解并输出电压，可实现后续稳定运行；与空气阴极电池相比，生物阴极电池产电较低，但对阳极基质的利用率较高。

毕业论文关键词：微生物燃料电池，启动期，环境因子，生物阴极

毕业设计说明书（论文）外文摘要

During startup and operation of microbial fuel cells (MFCs), operating environment factors affect performance of the reactors. The study aims at investigating the operating characteristics of the MFCs in start-up period, and the operation parameters (anodic and cathodic) on performance of the reactor system, in order to provide indication for subsequent stable operation.

Double chamber MFCs in triplicate were used in this study. Variations of voltage output, pH, temperature, conductivity, and ORP during the start-up period were examined. Statistical analysis software SPSS 13.0 were utilized for correlation analysis of the electricity production and environmental factors. Besides, nitrophenol reduction was studied with bio-cathode MFCs.

Results showed that anodic conductivity and cathodic ORP were significantly correlated to voltage output of the MFCs. pH of the anode and cathode showed highly significant correlation with voltage output of the MFCs. Thus pH is the key point in MFC operation. During the startup process of the biocathode, the nitrophenol degradation and voltage generation can be achieved, and the following stable operation is promising. Compared with the air cathode, the anodic substrate utilization is higher and voltage is lower in the biocathode MFC.

Keyword: microbial fuel cell, start-up period, environmental factors, biocathode

1 绪论 5

1.1 微生物燃料电池（MFC）简介 5

1.2 MFC的研究现状 6

1.3 MFC运行环境因子 9

1.4 MFC的生物阴极 10

1.5 立题依据及研究意义 10

2 实验部分 10

2.1 主要实验试剂与仪器 10

2.2 实验方法 13

3 结果与讨论 16

3.1电池启动中环境因子变化 16

3.2 环境因子与产电相关性分析 23

3.3 生物阴极电池启动期性能 24

结  论 29

致  谢 30

参考文献 31

1 绪论

1.1 微生物燃料电池（MFC）简介

微生物燃料电池（Microbial Fuel Cell，MFC）是一种利用微生物将有机物中的化学能直接转化成电能的装置。其基本工作原理是：在阳极室厌氧环境下，有机物在微生物作用下分解并释放出电子和质子，电子依靠合适的电子传递介体在生物组分和阳极之间进行有效传递，并通过外电路传递到阴极形成电流，而质子通过质子交换膜传递到阴极，氧化剂(一般为氧气)在阴极得到电子被还原与质子结合成水[1]。