山梨酸钾抑制黄曲霉生长的遗传学效应
关键词：黄曲霉；山梨酸钾；抑菌作用；遗传学效应
Genetic Effects of Potassium Sorbate Inhibited The Growth of Aspergillus flavus
Abstract: Aflatoxin, metabolites of Aspergillus flavus, is one of most deadly known carcinogens. However, it is still lack of a reliable way to inhibit growth of Aspergillus flavus. Sodium benzoate as well as potassium sorbate is chemical preservatives widely used in food industry. This study is aimed to test the Genetic effects of potassium sorbate on the growth of Aspergillus flavus, which provided reference data for preventing peanut and other corps becoming mildewed in preservation process. Using Aspergillus flavus spores as experimental materials, which were cultured in solid culture medium; the germination number was counted, colony size were measured, colony color, the morphological characteristics of mycelium and the spores of offspring were observed, respectively; at last, the data got from above, was analyzed with SPSS 16.0 statistical software, which is to study the inhibitory effects of preservation on Aspergillus flavus spore and growth of the mycelium. The results showed that potassium sorbate significantly inhibit Aspergillus flavus, and the inhibitory effect was significantly related to the applied concentrations. When the concentration of potassium sorbate was achieved 0.2 g/mL, the inhibitory effect was reached significant level and the decorative effect change is showed significantly .But, the present study demonstrated that there is no genetic effects of potassium sorbate inhibited the growth of Aspergillus flavus. When the concentration was achieved with 1.0 g/mL of potassium sorbate, the inhibition of the offering germination of aflatoxin spore reached 90 %. At a given concentration, potassium sorbate(≥1.0 g/mL )is a more secure, more convenient and more effective fungistat to Aspergillus flavus.
Key Words：Aspergillus flavus；Potassium sorbate；Antibacterial Effect；Genetic Effects
目 录
摘 要 1
引 言 2
1 实验材料 3
1.1 时间与地点 3
1.2 实验材料、试剂和用品 3
1.3 实验仪器 3
2 实验方法 3
2.1 培养基的配制 3
2.2 倒平板 4
2.3 黄曲霉孢子悬液的制备 4
2.4 防腐剂的抑菌实验 4
2.5 实验数据统计方法 5
3 结果与分析 5
3.1 不同浓度山梨酸钾对黄曲霉孢子后代萌发的影响 5
3.2 不同浓度的山梨酸钾对黄曲霉孢子子代菌落生长影响 6
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