除草混剂对野生大豆生长影响及噻二唑衍生物的作用研究
关键词：野生大豆；除草混剂；噻二唑衍生物；生理特性
Effect of herbicide mixed preparations on Wild Soybean Seedlings and the Research of 1,3,4-Thiadiazole Derivative on its Role
Abstract：Using wild soybeans “ZS” as experiment material, effects of the different concentration (0, 5, 10 and 15mg/L) of N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) on the growth of wild soybean seedling under herbicide mixed preparations stress was studied by sand culture. The results showed as follow: （1）under herbicide mixed preparations stress, the height of seedling and fresh weight were significantly decreased, while the content of chlorophyll, soluble proteins and soluble sugar were significantly decreased than those of control. The contents of proline and MDA were significantly increased(P<0.01).(2) Exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole-2-yl]-N'- 4-Nitrobenzoyl Urea (TNU) application on the leaf significantly increased the height of seedling and fresh weight, and the contents of chlorophyll, soluble proteins, soluble proline were significantly increased(P<0.01). The content of malonaldehyde (produce of membrane lipid peroxidation) was significantly decreased(P<0.01). The results showed that exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) could effectively mitigate the harmful effects from herbicide mixed preparations stress on plants, and promote the growth of wild soybeans, and the effect of 10mg/L TNU was optimal.
Key Words：1,3,4-thiadiazole; herbicide mixed preparations; Soybean; Physiological characteristics
目 录
摘要 1
ABSTRACT 1
引言 2
1材料与方法 2
1.1试验材料 2
1.2试验方法 3
1.2.1材料处理 3
1.2.2 测定项目与方法 3
1.3数据分析 4
2结果与分析 4
2.1 TNU对除草混剂胁迫下野生大豆幼苗苗高的影响 4
2.2 TNU对除草混剂胁迫下野生大豆幼苗苗鲜重的影响 4
2.3 TNU对除草混剂胁迫下野生大豆幼苗叶片内叶绿素含量的影响 5
2.4 TNU对除草混剂胁迫下野生大豆幼苗叶片内可溶性蛋白含量的影响 6
2.5 TNU对除草混剂胁迫下野生大豆幼苗叶片内脯氨酸含量的影响 7
2.6 TNU对除草混剂胁迫下野生大豆幼苗叶片内可溶性糖含量的影响 8
2.7 TNU对除草混剂胁迫下野生大豆幼苗叶片内MDA含量的影响 9
3讨论 10
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