不同光照条件对苦荞萌发的影响
毕业论文关键字：光质；荞麦萌发；黄酮；芦丁；DPPH
Effects of different light conditions on thegermination of Buckwheat
Abstract:This paper summarizes the composition and synthetic approaches of flavonoids in
buckwheat, as well as the role of some enzymes in biological metabolism synthesis.In this
experiment, color film and ultraviolet lamp of modulated wavelength were used to produce
different light quality for treating germinate tartary buckwheat. Effects of light quality(red light,
yellow light, green light, white light, blue light, UV-A(356nm) and UV-C(254nm))on flavonoids,
antioxidant properties and relative enzymes of flavonoids in germinated tartary buckwheat were
investigated, and dark was set as Compared with control. To knew the buckwheat flavonoids
contentIn in the process of germination, and the influence of related enzymes.
L－ phenylalaninammonia-lyase and chalcone isomerase are key to the synthesis of the
flavonoids in buckwheat. all the seven light quality significantly increased the content of total
flavonoids and rutin, and DPPH radical scavenging activity. UV-C had the highest influence on
them than any other light, and red light had the minimum influence on the L －
phenylalaninammonia-lyase, chalcone isomerase and flavonoids. Effectsof light quality on key enzymes (PAL, CHI) of flavonoids biosynthesis were similar to them on total flavonoids. Green
light did most favor for increasing RDEs activity and quercetin content.
Keywords: light quality; buckwheat; flavonoids; rutin; DPPH
目 录
. 绪论1
1.1 概述..1
1.1.1 苦荞麦概述1
1.1.2 苦荞麦营养和药用价值及应用开发..1
1.2 苦荞麦中黄酮类化合物及合成代谢中相关酶的概述2
1.2.1 黄酮类化合物研究进展.2
1.2.2 苯丙氨酸解氨酶（PAL）研究进展.4
1.2.3 查尔酮异构酶（CHI）研究进展..4
1.2.4 芦丁降解酶（RDEs）研究进展.4
1.2.5 光质对植物萌发的影响.4
1.3 本课题的研究的目的和意义.5
实验材料与方法..5
2.1 实验材料与试剂.5
2.2 仪器与设备..6
2.3 实验方法.7
2.3.1 苦荞萌发7
2.3.2 粗酶液提取..7
2.3.3 苯丙氨酸解氨酶（PAL）活性测定7
2.3.4 蛋白含量测定.7
2.3.5 查尔酮异构酶（CHI）活性的测定8
2.3.6 芦丁降解酶（RDEs）活性的测定..8
2.3.7 苦荞总黄酮类含量测定9
2.3.8 DPPH的测定9
实验结果与讨论10
3.1 牛血清标准蛋白曲线制作.10
3.2 不同光质对苯丙氨酸解氨酶（PAL）的影响.11
3.3 不同光质对查尔酮异构酶（CHI）的影响.. 12
3.4 不同光质对芦丁降解酶（RDEs）的影响.13
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