低剂量吡虫啉对小麦SOD、CAT酶活性及丙二醛含量的影响
毕业论文关键词： 吡虫啉；小麦；SOD；CAT；MDA
Effects of low doses of Imidacloprid on superoxide dismutase、catalase activity and malondialdehyde content on wheat
Abstract: Meanwhile pesticides to control pests on crops will produce a certain stress, which affects plant physiology and biochemistry. In this paper Yangmai158 as experimental materials to study the solution treated with different concentrations of imidacloprid change on wheat SOD, CAT activity and MDA. The results show that under continuous stress of low doses of imidacloprid, wheat there is no change in CAT activity time effect, SOD activity showed a time of the effect, compared with the control, wheat 0.2ppm concentration of imidacloprid treatment after 28 days, SOD activity was significantly higher than the control; under continuous stress of low doses of imidacloprid, MDA concentration showing the first decline after rising trend. Compared with the control, after 22 days after imidacloprid treatment, MDA levels were significantly decreased, 28 days after treatment, in addition to 0.0001ppm, 0.001ppm concentrations, the imidacloprid treatment significantly promoted the wheat produced in vivo MDA.
Keywords: Imidacloprid; wheat; superoxide dismutase; catalase; malondialdehyde
目录
一、绪论 1
（一）课题研究背景 1
（二）文献综述及研究意义 1
1、 化学农药对植物药害方面的研究 1
（1） 农药对作物主要营养物质的影响 1
（2） 对作物次生物质及次生代谢的影响 2
2、 逆境对植物酶保护系统的影响 2
3、 吡虫啉对植物生理生化指标的影响 3
4、 目前国内外有关低剂量化学农药对植物产生影响的研究报告存在的不足 3
5、 研究目的及意义 4
二、材料和方法 5
（一）基本材料 5
1、 实验场所及小麦品种 5
2、 供试农药 5
3、 供试仪器及设备 5
(二)方法 5
1、 实验设计 5
2、 样品蛋白质的含量测定 6
3、 SOD活性的测定 7
4、 CAT活性的测定 9
5、 丙二醛含量的测定 11
（三）酶活性及丙二醛含量测定试剂来源 13
（四）统计分析方法 13
三、结果与分析 14
（一）小麦SOD活性对低剂量吡虫啉胁迫的时间效应 14
（二）低剂量吡虫啉胁迫对小麦CAT酶活性影响 15
（三）低剂量吡虫啉胁迫对小麦MDA含量的影响 16
小结与讨论 17
致谢 18
参考文献 19
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