稻瘟病菌蛋白磷酸酶MoPtp1, MoPtp2的功能分析
毕业论文关键词：稻瘟病菌； 蛋白磷酸酶；致病性；MoPtp2
Functional Analysis of Phosphorase MoPtp1 and MoPtp2 in Magnaporthe oryzae
Abstract：Rice blast caused by Magnaporthe oryzae is one of the important diseases of rice. Rice blast can lead to a significant reduction in rice yield and a serious threat to food security in China. Thestudies of Saccharomyces cerevisiae have reported that Ptps is a class of protein phosphatase involved in MAPK feedback regulation, but its function in the filamentous fungi is still rarely reported, to analyze the protein phosphatase MoPtp1, MoPtp2 in rice blast fungus growth and development Pathogenicity of the protein phosphatase MoPtp1, MoPtp2 was genetically knocked out by homologous recombination, and the phenotypic analysis was carried out. The results showed that the growth of the mutant ΔMoPtp2 showed slowed down, and the sporulation and pathogenicity were significantly decreased compared with the wild type Guy11，nevertheless, ΔMoPtp1 had no significant changes in these ways . The resultsshwed that the growth, sporulation and pathogenicity of the complementary strains were consistent with Guy11 ,which is obtained by using the PEG-mediated method to These results show that MoPtp1 and MoPtp2 control the growth and pathogenesis of Magnaporthe grisea by regulating the synthesis of protein phosphatase. Theses results of the study have important theoretical significance for deeply understanding the pathogenic molecular mechanism of rice blast fungus and the development of potential targets for the development of new pesticides.
Key words: Magnaporthe oryzae；Protein phosphatase；pathogenicity；,MoPtp2
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1.1材料...2
1.1.1项目采用的菌株和培养基2
1.1.2项目采用的酵母及其培育条件3
1.2 方法3
1.2.1构建目的基因敲除载体3
1.2.2稻瘟病菌的原生质体的转化3
1.2.3目的基因序列与pYF11载体的酵母转化3
1.2.3.1制备酵母感受态细胞.4
1.2.3.2 酵母转化4
1.2.4酵母质粒转化大肠杆菌4
1.2.5 互补菌株的获得4
1.2.6供试菌株营养生长的测定....4
1.2.7供试菌株产孢量的测定4
1.2.8供试菌株致病力的测定4
2 结果与分析.4
2.1敲除载体与互补载体的构建.... ..5
2.2 MoPtp1与MoPtp2不参与调控营养生长...5
2.3 MoPtp2调控稻瘟病菌的产孢过程.....6
2.4 MoPtp1与MoPtp2参与调控稻瘟病菌的致病力...6
3 讨论7
4致谢.8
参考文献.8
稻瘟病菌蛋白磷酸酶MoPtp1, MoPtp2的功能分析