DL-蛋氨酸对IUGR哺乳仔猪肝脏糖代谢
毕业论文关键词：DL-蛋氨酸；宫内发育迟缓；肝脏；糖代谢；
Effects of DL-Methionine on hepatic glucose metabolism in IUGR suckling piglets
Abstract：This experiment was to study DL-methionine on hepatic glucose metabolism of intrauterine growth retardation (IUGR) suckling piglets. 5 normal birth weight (NBW) piglets and 10 IUGR piglets were pided into three treatment group (n = 5/group): NBW-CON group (NBW piglets fed based artificial milk), IUGR-CON group (IUGR piglets fed based artificial milk) and IUGR-MET group (IUGR piglets fed based artificial milk + 0.12% DL methionine). The piglets were slaughtered for samples at 21 d of age. Results showed that: (1) Compared with NBW-CON group, the phosphoenolpyruvate carboxykinase and glucose-6-phosphatase activities were significantly higher than IUGR-CON group (P < 0.05); (2) Compared with NBW-CON group, the glycogen synthetase activity of IUGR-CON group was mildly decreased (0.05 ≤ P < 0.1); (3) Compared with NBW-CON group, the mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphataseof IUGR-CON group were significantly increased (P < 0.05), and glycogen synthetase mRNA levels were significantly reduced (P < 0.05); Compared with IUGR-CON group, the mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase of IUGR-MET group were significantly increased (P < 0.05), and the activities of these two enzymes were mildly reduced (0.05 ≤ P < 0.1).These results show that DL- methionine may have ability to improve hepatic gluconeogenesis metabolism in IUGR suckling piglets.
Keywords：DL-methionine; IUGR; liver; glucose; metabolism;
目 录
摘要 1
关键词 1
Abstract 1
Keywords 1
引言 1
1 材料与方法 2
1.1 试验设计 2
1.2 样品采集 2
1.3 测定指标及方法 2
1.3.1 肝脏糖代谢评估 2
1.3.2 mRNA表达量 3
1.4 数据统计与分析 3
2 结果与分析 4
2.2 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖原代谢的影响 4
2.3 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖酵解酶活力的影响 5
2.4 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏mRNA水平的影响 5
3 讨论 6
3.1 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖异生酶活力及mRNA水平的影响 6
3.2 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖原代谢的影响 7