鸡胚胎期肌纤维发育的研究+文献综述
毕业论文关键词：鸡；胚胎期；肌纤文；成肌因子；肉质
The research on the development of muscle fiber in chicken embryonic period
Abstract: The growth and development of chicken muscle fibers are closely related to the quality of muscle, the main underlying factors are muscle fiber type, density and diameter of muscle fibers,and the number of muscle fibers in the embryonic period has been determined.There are many factors that affect the characteristics of muscle fibers. The aim of this research is to study the developmental characteristics and differences of density and diameter of embryonic muscle fibers in embryos of different breeds and different embryos,and the genetic effects of Pax3, Pax7, Mhc, Myod, Myog and other genes on muscle fiber characteristics. In this study, white feather chicken and Sasso chicken were used as experimental materials, I took different embryos of different embryos at different embryonic stages from these materials,which was made into paraffin sections,and which was stained for some days,after that I observed it under a microscope and taken a clear picture for image analysis and statistical processing. The expression of related Myogenic factors was detected by PCR, in order to study the relationship between the expression of related Myogenic factors and early muscle fiber development.The results show that there have a great differences between the fiber types of two kinds of chicken,the main show is white feather chicken muscle fiber average density less than Sasso chicken,and the average diameter of muscle fiber greater than Sasso chicken.Pax3,Pax7, Mhc, Myod, Myog and other Myogenic factors have different expression patterns during embryonic growth and development of embryos and have played different roles in different periods. In the rigorous experimental process, this result shows that muscle fiber density and meat quality was significantly positive correlation, muscle fiber density, the more tender meat; muscle fiber diameter and tenderness was negatively correlated, it can be considered muscle fiber more fine, The more fresh meat.The analysis of Myogenic factors further confirmed that the development of muscle fibers is affected by myoblasts, which provides a theoretical gist to study the development of muscle fibers during embryonic development.
Key words: chicken; embryonic period; muscle fiber; Myogenic factor; meat
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