猪卵母细胞IVM和精子质量对胚胎发育影响的研究
毕业论文关键词：卵母细胞；精子获能；体外受精
The study of the effects of oocytes maturation and sperm quality on the developmental viability of pig embryos
Abstract：In the study,techniques including in vitro maturation of oocytes,capacitation of sperm ,in vitro fertilization and embryo culture were used to research the effects of different methods of capacitation of sperm, sperm viability and selection of oocytes on cleavage rate and embryo development .Results show that no significant difference in the cleavage rate of the same pig semen with different emollients(P>0.05),the morula rate was significantly higher in the Sanyuan pig+Capacitation fluid A than in the Sanyuan pig+Capacitation fluid B(P<0.05).Subsequently, semina from Xinhuai with different sperm viability were performed for the IVF. And results show that significant differences in cleavage rate and morula rate were observed in fertilized embryos between different sperm viability(P<0.05).Finally,the cleavage rate and morula rate in the COCs with less than 3 layers were significantly lower than in the COCs with more than 3 layers(P<0.05).
Key words: Oocytes;capacitation of sperm;in vitro fertilization
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1材料2
1.1.1仪器设备2
1.1.2卵母细胞、精液及相关试剂2
1.2溶液的配制2
1.2.1获能液A2
1.2.2获能液B2
1.3方法2
1.3.1卵母细胞的采集和体外成熟2
1.3.2成熟卵母细胞的处理3
1.3.3鲜精的处理和质量检测3
1.3.4体外受精和胚胎培养3
1.3.5观察多精入卵3
1.4统计与分析3
2结果与分析3
2.1不同猪种及获能液对胚胎发育的影响3
2.2精子获能前活率对胚胎发育的影响4
2.3卵丘-卵母细胞复合体的卵丘细胞层数对胚胎发育的影响4
3讨论 5
3.1不同猪种及获能液对胚胎发育的影响5
3.2精子获能前活率对胚胎发育的影响5
3.3卵丘-卵母细胞复合体的卵丘细胞层数对胚胎发育的影响6
致谢6
参考文献7
猪卵母细胞IVM和精子质量对胚胎发育影响的研究
引言 猪的胚胎工程已成为推动畜牧业发展不可或缺的一大研究领域，通过其可以生产转基因猪或基因组修饰猪，可以改良品种、培育抗病猪种、提高肉质。同时，因为猪的基因与人相似度高，所以猪的胚胎工程在医学界备受欢迎，能够制备人类疾病模型、进行器官移植、生产药物蛋白、研究发育机理等。为了提供大量优质的猪卵母细胞和胚胎，如何完善猪的体外繁殖技术成为研究重点。
在国外，猪体外受精后的囊胚发育率为20%，有的会达到44.4%[1]，目前国内猪卵母细胞体外受精后的卵裂率达40%以上[2]，但是关于囊胚发育率的研究统计很少报道。与牛羊的胚胎体外生产相比，国内外猪的胚胎体外生产效率太低，技术也不够成熟。而选择优质的成熟卵母细胞和精子是提高胚胎体外生产效率的途径之一。