摘    要 最初，核糖体蛋白L10（RPL10）仅仅被当作核糖体的组成部分之一。然而越来越多的研究表明，RPL10除了能在蛋白质翻译中起作用外，还有许多核糖体外的功能。

拟南芥（Arabidopsis thaliana）具有容易获得各种代谢功能的缺陷型等优点，在基因功能研究等方面是非常优良的材料。值得一提的是，拟南芥有三个不等效的RPL10基因：AtRPL10A，AtRPL10B，AtRPL10C（基因编号AT1G66580）。前期实验已经证明了PVX/AtRPL10A重组病毒接种本氏烟草后，由于PVX病毒诱导了本氏烟草内源基因（RPL10A同源基因）的沉默（Virus induced gene silence,VIGS），导致植株矮化及病斑加重，同时还对育性也有严重的影响。在这基础上，我们将构建好的PVX/RPL10C重组载体线性化，并接种本氏烟草，考察PVX/AtRPL10C是否也具有类似PVX/AtRPL10A对本氏烟草产生的症状。

实验结果表明：接种了PVX/AtRPL10C重组病毒的本氏烟草出现的主要变化是植株矮化以及叶片卷曲。接种PVX/AtRPL10C重组病毒的本氏烟草在经过21天的培养后平均株高为5。7厘米，而接种PVX空载体的本氏烟草的平均株高为8。8厘米。而在经过47天的培养后，矮化现象变得更加明显，前者的平均株高为12。1厘米，后者的平均株高为30。2厘米。90345

Abstract Initially, ribosomal protein L10 (RPL10) is just one of as part of the ribosome。 however,  a growing number of studies have shown that RPL10 besides play a role in protein translation, there are many functions outside of the ribosome。

Arabidopsis thaliana is a good material in gene function research, it has easy access to a variety of metabolic function defect type, etc; And arabidopsis thaliana have three not equivalent RPL10 gene, which have been named AtRPL10A，AtRPL10B，AtRPL10C（genetic code AT1G66580）。 Our previous experiments have proved that after vaccination PVX/AtRPL10A reassortant virus, PVX virus will induces gene silencing（Virus induced gene silence,VIGS）in Nicotiana benthamiana(N。B。)’s endogenous genes(RPL10A homologous genes)。 For this reason, will lead to more serious plant dwarf and the disease spot, at the same time also has a serious effect on fertility。 On the basis of this, we will examine whether PVX/AtRPL10C like PVX/AtRPL10A ,will have the same symptoms。

The experimental results show: the N。B。 which vaccinated PVX/AtRPL10A recombinant virus may appear obvious plant dwarf phenomenon and leaf blade curled up, but didn't impact on disease spot and fertility。 After 21 days of cμltivation，the N。B。 which vaccinated PVX/AtRPL10C reassortant virus, its average plant height is 5。7 cm。 And the N。B。 which vaccinated PVX empty carrier, its  plant height to an average of 8。8 cm。 Not only that, after the cμltivation of the 47 days, dwarf phenomenon becomes more obvious, The average plant height is 12。1 cm of the former, the latter of the average plant height is 30。2 cm。

毕业论文关键词：拟南芥； 本氏烟草；RPL10C基因；PVX病毒；基因沉默；体外接种

Keyword: Arabidopsis thaliana；Nicotiana benthamiana；RPL10C；PVX virus；Gene silencing； In vitro inoculation

目    录

目录 4

引言 5

1材料与方法 6

1。1 实验材料以及设备 6

1。2 实验方法 7

1。2。1 PVX/AtRPL10C质粒提取 7

1。2。2 质粒DNA线性化 7

1。2。3 重组病毒RNA的体外转录 7

1。2。4 接种PVX/AtRPL10C重组RNA病毒