拟南芥SEC6基因在主根细胞程序性死亡中的功能初步研究
毕业论文关键词：Exocyst复合体；SEC6；主根；细胞程序性死亡；乙烯信号途径
Preliminary Research of SEC6 Gene Function in Programmed Cell Death of Arabidopsis Primary Root
Abstract： Exocyst complex is a tethering factor that directs the tethered process of the vesicle to the target
membrane, which is prevalent in eukaryotes. It is known that exocyst complex participates in a variety of
important biological processes such as cell plate formation, auxin transport, cell polarity growth and cell
autophagy in model plant Arabidopsis. Programmed Cell Death (PCD) plays an important role in the
growth and development, morphological composition and biological defense of plants. In the previous
experiment, we found that mutations in the SEC6 gene lead to PCD in Arabidopsis thaliana root cells. To
understand more deeply the molecular mechanism of PCD in Arabidopsis thaliana root cells by mutations
in the exocyst complex subunit gene SEC6, we need to do more in-depth study. In this study, we used the
exocyst complex subunit SEC6 gene pollen-compensating mutant PRsec6-1 as a material, and obtain
double mutant PRsec6-1 rbohD and PRsec6-1 ein2-5 by hybridization, then dye with trypan blue, PI ,
DCFH-DA, TUNNEL to analysis the development phenotype of the primary root. We found that blocking
the reactive oxygen species pathway did not alleviate the phenotype of PCD in the main root cells, while
blocking the ethylene signaling pathway could alleviate the PCD phenotype. Indicating that the primary
root cell programmed death caused by gene mutate of exocyst complex subunit SEC6 may depend on the
ethylene signaling pathway.
Key words: Exocyst complex；SEC6；Primary Root；Programmed cell death；Ethylene signal pathway
目 录
摘要3
关键词3
Abstract3
Key words3
引言（或绪论）3
1 材料与方法4
1.1 实验材料 4
1.1.1 植物材料4
1.1.2 酶、试剂及各种化学药品4
1.2 实验仪器 5
1.3 实验方法 5
1.3.1 拟南芥的栽培与管理5
1.3.2 拟南芥总 DNA 的快速提取（Edward 法）5
1.3.3 引物设计及 PCR 扩增6
1.3.4 拟南芥的人工杂交6
1.3.5 主根表型观察6
1.3.6 细胞死亡及细胞程序性死亡（PCD）检测6
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