外源乙烯对黑麦草苗期淹水胁迫的缓释效应
毕业论文关键词：外源乙烯；黑麦草；淹水胁迫；抗氧化酶系统；细胞膜透性
The relief effect research of the exogenous ethylene on flooding stress in seedling period annual ryegrass
Abstract: Precipitation in the southern China is relatively more and uneven distribution within a year, the planting of annual ryegrass to the regular of ryegrass seedlings were usually met with continuous rainfall, effects of ryegrass seedling emergence, it is difficult to form a strong seedling before winter. The experiment was set up to deal with the concentration gradient of 3 exogenous ethylene, according to blade spraying method, to explore the effect of exogenous ethylene on the mitigation of ryegrass seedlings under waterlogging stress. Determination of ryegrass plant height, fresh weight. Determination superoxide dismutase enzyme (SOD), peroxidase (POD), catalase (CAT) and other related physiological indexes of antioxidant enzyme system. The objective of the experiment was to alleviate the effects of waterlogging stress on the optimal concentration of ethylene, and to analyze its intrinsic regulation mechanism in order to provide a theoretical reference for the growth of ryegrass.
Keywords: Exogenous ethylene; Annual ryegrass; Flooding stress; Antioxidant enzyme system; Cell membrane permeability;
目 录
摘要 1
关键词1
Abstract1
Key words1
引言2
1□材料与方法3
1.1□材料3
1.2□方法3
1.2.1□超氧化物歧化酶（SOD）活性测定 3
1.2.2□过氧化物酶（POD）活性测定 4
1.2.3□过氧化氢酶（CAT）活性测定 4
1.2.4□丙二醛（MDA）含量的测定 5
1.2.5□细胞质膜透性电导率的测定5
1.2.6□株高和鲜重的测定5
2□结果与分析5
2.1□外源乙烯对淹水胁迫下黑麦草超氧化物歧化酶（SOD）活性影响 5
2.2□外源乙烯对淹水胁迫下黑麦草过氧化物酶（POD）活性影响 6
2.3□外源乙烯对淹水胁迫下黑麦草过氧化氢酶（CAT）活性影响 7
2.4□外源乙烯对淹水胁迫下黑麦草丙二醛(MDA)含量影响 8
2.5□外源乙烯对淹水胁迫下黑麦草电导率的影响9
2.6□外源乙烯对淹水胁迫下黑麦草株高和鲜重影响 10
3□讨论与结论 12
3.1□讨论 12
3.2□结论 13
致谢14
参考文献15
外源乙烯对黑麦草苗期淹水胁迫的缓释效应研究
引言：南方水网密集，地下水位高，土壤粘重，农田生境条件复杂，黑麦草播种至出苗期往往会遇到连续降雨，导致黑麦草出苗受到淹水胁迫，难以在越冬前形成壮苗[1]。淹水状态下根系空气交换受阻，缺氧抑制有氧呼吸，有氧呼吸被无氧呼吸所取代，消耗大量可溶性糖，积累酒精，根系活力迅速衰竭，对水分和有机物的吸收和利用下降，形成生理干旱甚至饥饿[2]。南方地区降水多，如江苏地区历年降水量均值为1028.9mm，夏季（6-8月）降水量达到510mm，占到全年降水的一半，7月的降水量最高，达到208.7mm，从1960年到2010年极端强降水量平均在8.5mm/d[3]。中国南方平均每年出现4次暴雨[4]。淹水胁迫是一种非生物胁迫，淹水状态多发生在夏季雨后，淹水状态下黑麦草根系空气交换受阻，缺氧抑制有氧呼吸，造成代谢絮乱，而使植株形态、生理和代谢过程发生变化，对黑麦草生长不利。