安徽一猪场仔猪肠道大肠杆菌噬菌体的分离鉴定
毕业论文关键词：大肠杆菌噬菌体；大肠杆菌；仔猪腹泻
Isolation and Identification of Escherichia coli Phage from Piglets of Anhui Pig Farm
Abstract: In recent years, more and more attention has been paid to phage in the intestine. Studies have shown that the type and quantity of intestinal phages directly affect the physiological state or microbial flora in the intestine. In this study, Escherichia coli was isolated from the piglets (normal and diarrhea) and the intestine of the growing pigs. The Escherichia coli phage was isolated from the piglets before and after weaning, and the isolates were isolated and analyzed. Phage studies have found that some phages can be present before and after weaning. Some phages are present only before weaning. Some phages are weaned only after weaning and more phage appears after weaning, and the phage spectrum appears after weaning. In this study, the phage distribution of intestinal Escherichia coli in piglets was studied for the first time, which laid the foundation for further study on the intestinal health of piglet intestinal Escherichia coli.
Key words: Escherichia coli phage; Escherichia coli; Piglet diarrhea
目 录
摘要…1
关键词1
Abstract…1
Key words1
引言…1
1材料与方法…2
1.1试剂和仪器2
1.1.1试剂…2
1.1.2试剂的配置2
1.1.3耗材…2
1.1.4仪器…2
1.2试验方法…2
1.2.1采样和处理2
1.2.2噬菌体的分离和纯化2
1.2.3噬菌体的保存…3
1.2.4点滴法测定宿主谱…3
1.2.5酶切…3
1.2.6提噬菌体基因组3
1.2.7噬菌体电镜观察4
2 结果与分析…4
2.1噬菌体的初步分离 …4
2.2噬菌体的宿主谱测定 5
2.3噬菌体的酶切图谱分析 …6
2.4电镜观察噬菌体形态 7
3 讨论8
3.1仔猪肠道噬菌体的分布 …8
3.2噬菌体宿主谱的鉴定 8
3.3噬菌体的分离和保存 8
致谢9
参考文献 9
安徽一猪场仔猪肠道大肠杆菌噬菌体的分离鉴定
人和动物肠道内的微生物区系是地球上最高密度、最复杂的生态系统之一，每克粪便中细菌的数量可高达1013。噬菌体（bacteriophage, phage）是寄生于细菌、霉形体、螺旋体、放线菌以及蓝细菌等的一类病毒，亦称细菌病毒。在自然界分布极广，凡是有上述各类微生物的地方，都有相应种类噬菌体的存在，其数目与寄主的数量成正比[1]。噬菌体是地球上增殖能力最强，数量最多的物种之一，它存在于任何有细菌的地方，影响着各种生态系统中细菌的数量。近几年，肠道内噬菌体和微生物菌群之间的关系受到越来越多的关注。噬菌体与细菌间的“捕食效应”使它们之间存在着动态变化关系，噬菌体的种类和数量直接影响着肠道内的生理状态或微生物区系[2] 。大肠杆菌在仔猪阶段对仔猪的影响十分巨大，因为大部分仔猪腹泻都是由大肠杆菌引起的。那仔猪肠道的大肠杆菌噬菌体对大肠杆菌有影响吗？Golomidova 等 (2007)研究发现，马的粪便中大肠杆菌的多样性与大肠杆菌噬菌体感染的选择压力有关[3]。所以本试验将首次以仔猪肠道大肠杆菌噬菌体为研究对象，研究大肠杆菌噬菌体在仔猪肠道的分布规律，为将来研究肠道大肠杆菌噬菌体对大肠杆菌的影响奠定基础。