日粮氮源与乳酸链球菌素对湖羊瘤胃发酵血液生化及氮平衡的影响
毕业论文关键词：乳酸链球菌素；湖羊；瘤胃发酵；血液生化；氮平衡
Effects of Nitrogen Source and Nisin on Rumen Fermentation, Blood Metabolites and Nitrogen Balance of Hu sheep
Abstract: This test is to add by feeding experiment study nisin of the two sheep rumen fermentation under the conditions of different nitrogen source, blood biochemistry and the influence of nitrogen metabolism. The two factors were designed with 2 by 2 factors, and the two factors were the nitrogen source (soybean meal, DDGS) and lactate streptococcus (0, 30.5 mg/kg), and the four kinds of daily grain. Select 32 only 20 kg of weaning male sheep, according to randomized block design and with the principle of equal size, according to the weight is pided into four, respectively fed diet accordingly, each processing 8 sheep, single bar feeding, free drinking water. The experiment was based on the 46:54 (DM base) total mixed daily grain (TMR) as the base day food, 10 weeks in the trial period, and the first two weeks were the adaptation period. The group C based diet fed soybean meal as nitrogen source, group D fed soybean meal as nitrogen source to add 30.5 mg/kg Nisin, treatment group A and B in the add DDGS as nitrogen source based diet added respectively 0 mg/kgNisin and 30.5 mg/kgNisin. The amount of consumption was measured in the eighth week of the trial period. In the 8th week, the samples were collected for 3 days and used for the determination of nitrogen metabolism. The gastric juice was collected from the gastric juice of all the lakes, and was used for the analysis of fermentation parameters after feeding on the 6th week of the experimental period and the following morning of the 8th week. 6 and 8 weeks of the trial period, in the morning between 3 h after feeding on sheep all trials in jugular vein quantitative blood 10 ml, 15 minutes 3000 RPM centrifugal separation of serum, serum on - 20 ℃ to save to the analysis, then you can get blood analysis data. The result shows: the soybean meal protein rumen degradation rate is high, rumen DDGS protein degradation rate is low, and streptococcus element to sheep rumen fermentation had no significant influence (P > 0.05), and has a significant effect on sheep blood biochemical (P < 0.01), blood glucose, albumin, total protein, etc all have certain difference. In the case of nitrogen metabolism, the results showed that the rate of degradation of the soybean meal was high, the degradation rate of the DDGS was low, and the substitution of DDGS for soybean meal to reduce the nitrogen discharge. The nisin can inhibit the removal of amino acids from the rumen and improve the efficiency of nitrogen.
Key words: Nisin; Hu sheep; Rumen fermentation; Blood biochemical; Nitrogen balance
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