盐胁迫下大岛野路菊形态及组织结构的变化
毕业论文关键词：盐浓度；形态变化；叶片增厚；叶片形态变化
Changes in morphology and organizational structure of chrysanthemum crassum under salt stress
Abstract： The seedlings of chrysanthemum crassum were cultivated with with 0 (control),80,120and 200 mM NaCl in Hoagland aqueous solution and observed and recorded its growth. The results showed that compared with the control, salt stress had an inhibitory effect on the growth of chrysanthemum crassum, but there was no significant difference in plant growth under different concentrations of salt. By comparison with the control and 120 mM NaCl stress ，Salt stress will lead to its plant height becomes low, stem internode shorter, the middle of the full expansion of leaf thickening, lower leaves withered and other changes. In-depth study found that the leaves of chrysanthemum crassum were significantly thickened with the prolongation of salt stress, and was due to cell enlargement. Also occurred in leaf epidermal cells, palisade tissue cells, petiole cells increased, stomatal density smaller, epidermal hair loss, gland rupture and so on.
Key words: Salt concentration；Morphological changes；Leaf thickening ；Leaf morphological changes
目 录
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）1
1材料与方法2
1.1材料 2
1.2材料培养方法2
1.3整株形态变化的记录2
1.4叶片大小的测量2
1.5石蜡切片制作2
1.6叶片气孔、表皮细胞以及栅栏组织细胞的观察2
1.7扫描电镜观察3
1.8数据分析3
2结果与分析3
2.1大岛野路菊在不同盐浓度下的生长情况3
2.2大岛野路菊叶片在盐胁迫下的形态变化5
2.3盐胁迫下大岛野路菊叶片表面细胞大小及气孔密度的变化7
2.4盐胁迫下大岛野路菊叶片表面微观结构的变化9
2.4.1盐胁迫下大岛野路菊叶片表面表皮毛密度的变化9
2.4.2盐胁迫下大岛野路菊叶片表面腺体的变化9
2.5盐胁迫对大岛野路菊叶柄及幼茎结构的影响10
3讨论 11
致谢12
参考文献12
盐胁迫下大岛野路菊形态及组织结构的变化
引言 盐胁迫通常会导致植株的形态变化，由于盐胁迫对光合的抑制和对呼吸需求的提高，会对其长势造成影响[1]；植株为了保持生长，会将生长部位的盐分转移到衰老组织中[2]，从而会导致老叶变黄、脱落等；在盐胁迫下，耐盐植株体内会积累水分以供细胞正常运转与稀释盐分，便会导致植株叶片、茎等器官的增厚，如毛白杨在盐胁迫下叶片显著[3]，盐地碱蓬和假红树等在盐胁迫下发生叶片与茎干的增厚[4]。为防止水分流失，植株在盐胁迫下一般会选择关闭气孔，而且盐胁迫会影响植株叶片生长过程中气孔的分化，从而导致叶片气孔密度的降低，如三色苋在盐胁迫下其新生叶片的气孔密度显著降低[5]。部分植物如木槿等可以通过腺体破裂与表皮毛脱落等主动排出盐分[6]。
而在本实验，主要通过观测大岛野路菊在盐胁迫下整株及叶片为主的形态的变化，试图通过盐胁迫下发生的形态变化，揭示其与其耐盐性关系间的联系。
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