陆地棉SIZ1基因的电子克隆及其分析
毕业论文关键词： SIZ1；SUMO；陆地棉; 电子克隆
In Silico Cloning and Analysis of GhSIZ1 Gene
Abstract：SUMO (small ubiquitin-related modifier) modification is one of the important ways of intracellular protein function adjustment, it is widely exists in eukaryotic highly conserved protein family, there are three type gene in vertebrates. SIZ1 in cold resistance, heat resistance, drought resistance, phosphate starvation response, antiviral defense, flowering regulation and physiological processes plays an important role in plant. This paper mainly using bioinformatics methods, the use of Arabidopsis thaliana AtSIZ1 gene to the upland cotton in electronic cloning is not retrieved clear results. Using Arabidopsis amino acid or nucleotide sequences in Raymond's cotton, cotton in Asia than in the database retrieval sequence, the two are in series consequent repetition. Use Raymond's cotton, cotton siz1a Asia/b amino acid or nucleotide sequences in upland cotton ESTs database than the retrieval for nine sequences, four sequence alignment results are exactly the same. Use CAP3 Sequence Assembly Program of upland cotton 5 'end and 3' end ESTs were assembled obtained GhSIZ1 gene 5 'end and 3' end ESTs nucleic acid Sequence. Using bioinformatics methods to further its DNA base sequence and protein amino acid sequence was analyzed, and aims at providing a better study SIZ1 gene with certain theoretical data.
Key Words: SIZ1; SUMO; Gossypium hirsutum; Cloning in silico
目 录
摘 要 1
引言 2
1陆地棉SIZ1电子克隆方法与步骤 6
1.1获得Atsiz1的基因和蛋白序列 6
1.2直接在陆地棉ESTs库中blast获得EST片段 6
1.3获得雷蒙德氏棉、亚洲棉SIZ1基因序列 6
1.4获得陆地棉EST片段 6
1.5获得陆地棉GhSIZ1基因核苷酸序列 6
2陆地棉SIZ1电子克隆的结果与分析 7
2.1Atsiz1的基因和蛋白序列 7
2.1.1 Atsiz1基因核苷酸序列 7
2.1.2 Atsiz1基因的蛋白质序列 7
2.2雷蒙德氏棉、亚洲棉数据库中比对获得检索序列 8
2.2.1 亚洲棉Gasiz1a核苷酸序列 8
2.2.2亚洲棉Gasiz1a氨基酸序列 8
2.2.3亚洲棉Gasiz1b核苷酸序列 9
2.2.4亚洲棉 Gasiz1b氨基酸序列 9
2.2.5雷蒙德氏棉Grsiz1a基因核苷酸序列 10
2.2.6雷蒙德氏棉Grsiz1a基因氨基酸序列 10
2.2.7雷蒙德氏棉Grsiz1b基因核苷酸序列 10