番茄茉莉酸信号传导途径关键酶的序列特性和聚类分析
毕业论文关键词：番茄；茉莉酸；信号传导；关键酶
Sequence Feature and Clustering Analysis of the Key Enzymes from Jasmonic Acid Signal Transduction in Tomato
Abstract: Jasmonic acid hormone includs jasmonic acid and methyl jasmonic acid. jasmonic acid was firstly isolated from the fungus broth, and methyl jasmonic acid is the component of flower aroma. JAs plays an important role at all stages of plant growth and development, and make defense reaction of biological and abiotic stress. JA regulates plant growth, reproduction, responding to environmental stresses, especially the role of the defense reaction of herbivorous insects persity, so the signal pathways in JA ability has very important significance to improve plant growth and plant resistance. So far, having learned that the important core gene jasmonic acid signal transduction pathways are COI1, JAZ, MYC. However, previous studies mostly focused on jasmonic acid biosynthesis, expression regulation and control mechanism, etc.In the ways of jasmonic acid signal transmission of the receptor, the substrate and ways of jasmonic acid signal other important components in the aspects of understanding is not very detailed, and have not do analysis of the key enzyme of the signal pathway system , this study applies the method of bioinformatics to key enzyme in tomato jasmonic acid signal transmission in detail.
Key words: Tomatoes, Jasmonic acid, Signal transduction, Key enzyme
目 录
摘 要 1
Abstract 1
引言 2
1.数据与方法 4
1.1 数据来源与序列检索 4
1.2 基因结构分析 4
1.3 蛋白序列特征分析 4
1.4 序列比对与聚类分析 5
1.5 同源基因对的表达分析 5
2.结果与分析 5
2.1 番茄茉莉酸信号传导途径关键基因的鉴定 5
2.2番茄茉莉酸信号传导途径关键基因的结构特点 5
2.3番茄茉莉酸信号传导途径关键酶保守基序与功能结构域 6
2.4番茄茉莉酸信号传导途径关键酶的聚类结果 7
2.5同源基因对的表达情况 9
3.讨论与结论 10
参考文献 11
致谢 13
番茄茉莉酸信号传导途径关键酶的序列特性与聚类分析
引言
植物激素是植物细胞分泌的微量活性有机物，来应对特定的外界环境，在调控植物发育、繁殖以及对环境胁迫做出反应，特别是对食草昆虫的防御反应起着重要的作用，它们在植物的各种生长发育和环境的应答中扮演着十分重要的角色[1]。近年来，人们对植物激素的生物合成、作用机理和生理作用等方面都进行了深入的探究，并对其信号传导及调控、激素信号之间的相互作用都有了新的认识， 同时随着生物信息学的发展，对其结构和功能进行了鉴定和分析，这进一步促进了人们对植物激素的研究。要对植物激素的信号传导过程做充分的研究，首先要研究激素受体功能，生长素、赤霉素、脱落酸的受体和信号传导途径的研究已经有了很大的进展[2]。目前已经发现了2类生长素受体：生长素结合蛋白1和运输抑制剂响应1蛋白，前者介导生长素早期反应，后者介导晚期反应。而对赤霉素的已有研究表明，赤霉素诱导产生α-淀粉酶，赤霉素促进茎伸长反应的信号传导途径也已有深入的了解。不同激素信号传导途径均有不同，本文主要对茉莉酸信号传导途径进行介绍[3]。