聚赖氨酸作为化妆品防腐剂可行性分析
关键字： 聚赖氨酸；尼泊金甲酯；化妆品；抑菌效果;毕业论文
Analysis of ε-Polylysine as a cosmetic preservative Feasibility
Abstract：The use of natural biological preservative is becoming the current research and development trend for cosmetic preservative. The advantages of polylysine (ε-poly-L-lysine, EPL) as a biological preservative include broad inhibition spectrum, high water-solubility, no toxic side effects and high safety. This study examines the antibacterial properties of polylysine against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Aspergillus niger; determines the corresponding MIC values; and tests cosmetic preservative efficacy. The experimental results show that: while polylysine has superior antibacterial compares to commonly used cosmetic preservative agent methylparaben, its inhibitory effect on fungi is slightly worse. To optimize preservative effect, the study proposes the use a mixture of polylysine and methylparaben in 3:1 ratio by weight at 0.05%. The study provides theoretical and practical basis for polylysine to be used as a natural alternative to chemical preservatives such as methylparaben while maintaining cosmetic preservative safety.
Keywords: Polylysine; Methylparaben; Cosmetics; Bacteriostatic effect
目 录
1 绪论 1
1.1 化妆品防腐剂简介 1
1.1.1化妆品用防腐剂的要求 1
1.1.2化妆品防腐剂使用现状 1
1.2 聚赖氨酸简介 2
1.2.1 聚赖氨酸的结构 2
1.2.2 聚赖氨酸的理化性质 3
1.2.3 聚赖氨酸的抑菌机理 3
1.2.4 聚赖氨酸的生物学性质 3
1.2.5 聚赖氨酸的发酵制备 4
1.2.6聚赖氨酸作为防腐剂国内外研究现状 4
1.3本课题研究的主要内容 4
2.1 材料 5
2.1.1 供试菌种 5
2.1.2 培养基和主要试剂 5
2.1.3 主要器材设备 6
2.2 方法 6
2.2.1供试菌样制备： 6
2.2.2试剂制备 6
2.2.3 测试平皿制作 6
2.2.4 滤纸片法测定防腐剂抑菌效果 6
2.2.5 MIC的确定 7
2.2.6供试化妆品的制备 7
2.2.7防腐剂效力测试 8
3 实验结果与分析 9
3.1防腐剂抑菌效果实验观察结果 9
3.1.1 防腐剂对大肠杆菌的抑菌效果 9
3.1.2 防腐剂对金黄色葡萄球菌的抑菌效果 10
3.1.3 防腐剂对铜绿假单胞菌的抑菌效果 10
3.1.4 防腐剂对黑曲霉的抑菌效果 11