浸叶法粘虫对杀虫剂的敏感性测定
毕业论文关键词：粘虫；杀虫剂；敏感性；抗性水平
Sensitivity of Mythimna separata to Insecticides in Jiangsu and Anhui Provinces
Abstract： The susceptibility of Mythimna separata (Walker) from Jiangsu and Anhui Provinces to five commonly used insecticides and the virulence of phoxim to two field populations were tested by leaf dipping method in laboratory. The results showed that compared with the susceptible strain of M. separata，the two field populations had developed varied resistance to lambda-cyhalothrin, beta-cypermethrin and chlorantraniliprole, and no obvious resistance to emamectin benzoate and chlorpyrifos was observed in the two populations tested. The order of resistance levels of armyworm in Jiangsu was as follow: lambda-cyhalothrin> beta-cypermethrin> chlorantraniliprole> emamectin benzoate> chlorpyrifos, The resistance multiple is in turn 38.2, 12.0, 6.4, 0.9, 0.4, The order of resistance level of armyworm in Anhui was as follow: chlorantraniliprole> lambda-cyhalothrin> beta-cypermethrin> emamectin benzoate> chlorpyrifos, The resistance levels were 47.6, 13.8, 9.3, 2.1, 1.4 respectively. Phoxim on armyworm in the two field populations have shown a strong virulence, the LC50 were 5.725 mg / L and 4.404 mg / L, respectively. It was suggested that alternative and rational application of different insecticides is urgently required to delay the development of insecticide resistance．
Key words: Mythimna separate; insecticide, insecticide resistance; insecticide susceptibility
目 录
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）1
1材料与方法2
1.1供试材料2
1.1.1供试昆虫2
1.1.1.1 幼虫饲养2
1.1.1.2 成虫饲养3
1.1.2 供试药剂3
1.2试验方法 3
1.2.1 浸叶法3
1.2.2 敏感性测定3
1.2.3 数据分析3
2 结果与分析4
2.1 不同杀虫剂对粘虫敏感种群的毒力4
2.2江苏粘虫种群对不同杀虫剂的抗药性
4 2.3安徽粘虫种群对不同杀虫剂的抗药性4
3 讨论 5
致谢6
参考文献7
表1 粘虫人工饲料配方2
表2 5种杀虫剂对粘虫敏感品系3龄幼虫的毒力4
表3江苏粘虫3龄幼虫对杀虫剂的抗性水平4
表4安徽粘虫3龄幼虫对杀虫剂的抗性水平5
江苏、安徽粘虫对杀虫剂的敏感性测定
引言
粘虫Mythimna separata（Walker），鳞翅目，夜蛾科，又俗称行军虫、夜盗虫和剃枝虫，为一种典型的远距离迁飞性害虫，亦是对我国粮食生产安全造成严重威胁的重大生物灾害[1]。其危害特点可分为以下几点：一是发生范围广，危害世代多。在我国，除新疆外的地方均可造成危害并暴发成灾，每年大约有4-5次大范围迁飞危害；二是危害的作物种类和组织多。可危害玉米、小麦和水稻等主粮作物及其它100多种作物，并可将啃光作物的叶片，咬断麦穗和谷穗进而造成严重减产和绝产；三是产卵隐蔽性强，且量大。粘虫成虫喜欢把卵产在作物端部以及下部的杂草、枯叶或叶鞘中，农民不易发现。雌虫的繁殖能力也非常强，每只雌蛾一生可以产1000-2000粒卵，最多可达3000粒[2]；四是爆发性明显，造成的产量损失严重。从1950-1989这40年间，粘虫有17年爆发成灾，成灾面积7191万hm2 ，损失粮食超过1643万吨[3]。2012年，3代粘虫在华北、东北的发生面积达397.4万hm2，多数省份危害程度居历史之最[4]；五是发生危害历史长。从公元482-1360年间，曾有35项粘虫暴发成灾的记录。从1970-1989这20年间，每10年就有6年大发生。1990以来，粘虫的局部危害也一直没有停止过，直至2012年全国粘虫的大发生[5]。