生物转化法制备天然香料丙位癸内酯的初步研究
本论文对市售17种高活性干酵母粉进行筛选，确定了“安琪高活性干酵母”为高产菌株，然后又对其发酵条件进行了优化，确定其最佳培养基为：葡萄糖0.5%、蓖麻油1%、吐温-80 0.8%、MgSO4 0.3%、NaCl 0.2%、Na2HPO4 0.12%、KH2PO4 0.1%；温度28℃，接种量0.1%，种龄48h，pH值自然。在此条件下，GDL的发酵产量达到476.3mg/L。
同时本文也对GDL的提取条件，气相检测条件和GDL生成速率进行了研究。5902
关键词： 酵母、蓖麻油、发酵、γ-癸内酯
The preliminary study of the biological preparation of γ-decalactone
Abstract：γ-decalactone(GDL) is a lactone, it has a wide application in the food, cosmetic and light industry fields, GDL nearly 10,000 tons per year worldwide products are basically using chemical synthesis. With the increasing demand for natural additives, urgent need to develop new production technology which may alternative the traditional technology. Biotransformation is the best way to get natural GDL.
We screened 17 kinds of high active dry yeast powders and determined “Angel active dry yeast” as the high - producing strain.We optimized the fermentation conditions and determined their best medium for: glucose 0.5%, castor oil 1%(vol/vol), tween-80 0.8%(vol/vol), MgSO4 0.3%, NaCl 0.2%, Na2HPO4 0.12%, KH2PO4 0.1%, temperature 28℃, seed age 48 hours, inoculation 0.5%, pH is natural.
We also studied GDL extraction conditions, test conditions and GDL generation rate.
Key words: yeast; castor oil; Fermentation; γ-decalactone
1 绪论…1
1.1 γ一癸内酯简介1
1.2 γ一癸内酯研究发展1
1.3 γ一癸内酯国内外研究近况…2
1.4 蓖麻油及其在γ- 癸内酯转化中的应用…2
1.5 生产天然γ一癸内酯的基本方法3
1.6 研究的意义3
1.7 研究内容…4
2 实验材料和方法…4
2.1 材料…4
2.1.1 菌种4
2.12 试剂5
2.1.3 实验主要仪器…5
2.1.4培养基…5
2.2 实验方法…6
2.2.1 丙位癸内酯转化生产工艺6
2.2.2 菌种筛选6
2.2.3 发酵培养基的优化6
2.2.4.γ- 癸内酯萃取7
2.2.5γ- 癸内酯定量检测7
3实验结果与讨论7
3.1菌种筛选7
3.1.1菌种初筛7
3.1.2菌种复筛9
3.2发酵培养基的优化…10
3.2.1 单因素实验10
3.2.1.1葡萄糖单因素10
3.2.1.2 蓖麻油单因素11
3.2.1.3玉米浆单因素12
3.2.1.4 表面活性剂单因素…13
3.2.1.5 吐温-80单因素…14
3.2.2正交优化实验…15
3.2.2.1 各项重要性比较15
3.2.2.2 显著性描述16
3.2.2.3 最佳方案的确定16
3.3 提取率的确定19
4 结论20
5 致谢21
6参考文献22
一 绪论1.1γ一癸内酯简介
γ一癸内酯(gamma—decalactone，GDL)．又名丙位癸内酯，化学名称为γ一己基一γ一丁内酯，是一种五元环内酯化合物，为天然桂花主要香气成分之一，广泛地存在于水果、饮料、肉制品和日常食品中，它以其诱人的桃香和低香气阈值的特性而在香料工业得到普遍应用。［1］。
上一篇：柚子皮对铬的吸收状况的研究