紫苏醛对白色念珠菌的细胞周期和细胞凋亡的影响
毕业论文关键词：紫苏醛；白色念珠菌；细胞周期；细胞凋亡
Influence ofperillaldehydeon cell cycle and apoptosis of Candida albicans
Abstract：Control the fungal infection caused byC.albicans is relatively difficult clinical problem. Consequently,research and development of new effective method of resistance of C.albicans has become one of the emphases on the antifungal drugs in recent yearsan,andperillaldehyde is a kind of effective, broad-spectrum, low toxicity of antibacterial drugs. Thisexperiment mainly study perillaldehyde solution mechanism of resistance of C.albicans,at different concentrations of perillaldehyde solution of C.albicans sterol content, cell membrane injury, in a simple discussion perillaldehyde on the mechanism of the fungal cell membrane. Ergosterol content is measured with a UV-spectrophotometer,usingflowcytometryinstrumentdetectioncell membrane damage, the results show that the perillaldehyde can be applied to the cell membrane, the synthesis of ergosterol in inhibition of cell membrane, change the permeability of membrane, damage the cell membrane. Using flow cytometry instrument testing analysis of cell cycle and cell apoptosis, test results indicate that, perillaldehyde can make the cell cycle arrest in GO/G1 phase, induction of cell apoptosis. In conclusion, different concentrations of perillaldehyde (0-0.4µL/mL) on the growth of C.albicans has inhibitory effect, inhibition effect and is directly proportional to the concentration, antifungal mechanism is mainly attributed to inhibit the synthesis of ergosterol in the cell membrane, destroy the integrity of the cell membrane, block the cell cycle and inducing apoptosis.
Key words：Perillaldehyde; C.albicans; Cell cycle; Apoptosis
目录
引言 1
1 材料与方法 3
1.1供试材料 3
1.1.1原料 3
1.1.2实验试剂与仪器 3
1.1.3 培养基 4
1.1.4 各种试剂溶液的配制 5
1.2 实验方法 6
1.2.1 白色念珠菌菌种活化 6
1.2.2 菌悬液的制备 6
1.2.3 微孔稀释法测定最低抑菌浓度(Minimum Inhibitory Concentration, MIC) 6
1.2.4紫苏醛对白色念珠菌甾醇含量的影响(Sterol Quantitation Method, SQM) 6
1.2.5紫苏醛对白色念珠菌细胞膜损伤的影响 7
1.2.6 紫苏醛对白色念珠菌细胞周期的影响 7
1.2.7 紫苏醛对白色念珠菌细胞凋亡的影响 8
2 结果与分析 9
2.1紫苏醛对白色念珠菌的MIC值 9
2.2紫苏醛对白色念珠菌甾醇含量分析 9
2.3紫苏醛对白色念珠菌细胞膜损伤测量 10