菌株SQR9野生型和突变体在不同IAA合成中间产物吲哚类物质中产IAA能力的探究
毕业论文关键词：解淀粉芽孢杆菌；突变体ΔysnE；IAA合成途径；产IAA能力
the study of Capacity of IAA synthesis from Bacillus amyloliquefaciens SQR9 and mutant in different IAA synthesis intermediates the indoles
Student majoring in Agricultural Resource and Environment WangMingliang
Tutor ZhangRuifu
Abstract：Bacillus amyloliquefaciens SQR9, isolated from the rhizosphere of cucumber, can control Fusarium wilt of cucumber. The strain was proved to be an outstanding plant growth-promoting rhizobacteria (PGPR) strain by its ability to inhibit the growth of Fusarium oxysporum f. sp. cucumerinum J. H. Owen (FOC) and decrease the incidence of Fusarium wilt in field experiments, and a bioorganic fertilizer made of organic fertilizer fermented with B. amyloliquefaciens SQR9 is widely used in agricultural production.Biological dependence on tryptophan synthesis of IAA in several ways, has its specific intermediate, according to the specific intermediate respectively named IPA, TAM, IAM,and IAN.This experiment through the solution of starch bud robe coli SQR9 and mutant ΔysnE, various ways of intermediate product is added to the analysis of SQR9 and ΔysnE fermented liquid of IAA and the seven key components, the ysnE to search for genes involved in IAA synthetic way. The conclusion of the experiment is:IPA and TAM approach may be the two main pathways of the SQR9 dependence on tryptophan synthesis IAA. Gene ysnE may participate in IAA respectively synthetic ways of indole pyruvate way and tryptamine way, and it is between indole pyruvate and indole acetaldehyde and serotonin function between indole acetaldehyde. Gene ysnE is not involved in IAM and IAN.
Key words: Bacillus amyloliquefaciens；mutantΔysnE；IAA synthesis pathway；Capacity of IAA synthesis
目 录
摘要 1
关键词 1
Abstract 1
Key words 2
引言 2
1 材料与方法 3
1.1 材料 3
1.2 方法 3
1.2.1 发酵液制备 3
1.2.2 发酵液样品处理 3
1.2.3 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中IAA定量分析 3
1.2.4 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中TOL和ILA定量分析 5
1.2.5 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中TAM定量分析 5
1.2.6 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中IAN和IAM定量分析 5
2 结果与分析 5