油菜TFIIIA型锌指蛋白的挖掘与鉴定
毕业论文关键词：油菜，TFIIIA型锌指蛋白，生物信息学分析
Mining and Identification of Rapeseed TFIIIA Type
Zinc Finger Protein
Abstract: the eukaryotic transcription factors roughly pided into zinc finger protein, helix-turn-helix, alkaline leucine-zipper four types. the zinc finger protein were pided into four subtype including C2H2 zinc finger, C2C2, C2HC, C2C2C2C2 and C2HCC2C2, Plant type TFIIIA belong to C2H2 zinc finger protein transcription factors, contains conservative QALGGH area, involved in plant growth and response to stress. By isolation and identification of gene induced by abiotic stresses, and further studying their function, a relatively detailed stress signal transduction network system gradually were formed. In the paper, A protein sequnence from Arabidopsis thaliana, named TFIIIA type zinc finger protein as a template, protein-blast was performed,using NCBI database to obtain homology sequences in rapeseed, we found 27 homologous sequences, the similarity between the 27 sequence and tmplete, of twelve sequence have over 28% similarity and the Query coverage is higher than 46%, article 12, their protein sequence length were different from 374 aa to 1325 aa, and hver 6-10 exons in gene sequence, according their domain, they were grouped three types, the first group include 9 sequences, they all have COG5408 conserved domain similar to tmplete sequence from Arabidopsis thaliana L.
Key words: Rapeseed, TFIIIA type zinc finger protein, Bioinformatics analysis
目 录
摘 要 1
Abstract 1
引 言 2
1.材料和方法 3
1.1油菜TFIIIA型锌指蛋白家族序列来源 3
1.2基因结构、基因定位信息 3
1.3分子量、等电点和保守结构域和亚细胞定位 3
1.4进化分析 3
2.结果与分析 4
2.1油菜TFIIIA型锌指蛋白基本信息 4
2.2油菜TFIIIA型锌蛋白家族的保守结构域分析 5
2.3油菜TFIIIA型锌指蛋白家族成员的序列比对与进化 6
3.讨 论 7
参考文献 9
致 谢 12
油菜TFIIIA型锌指蛋白的挖掘与鉴定引言
甘蓝型油菜(Brassica napus L.)，甘蓝型油菜是三种油用油菜(白菜型油菜、芥菜型油菜、甘蓝型油菜)中籽粒产量最高的种类，目前在我国长江中下游流域大量种植，是我国重要的油料作物。优势利用优质育种结合起来，但因育种方法不当或未找到理想的种质，至今进展不大。植物在生长发育过程中往往会遭受干旱、高盐及低温等非生物胁迫,植物在适应这些胁迫过程中产生了一系列从细胞到生理水平的应答反应,这些反应往往是通过基因表达的变化实现的。甘蓝型油菜中TFIIIA型锌指蛋白含有保守的QALGGH区，参与植物的生长发育和逆境胁迫应答，对油菜的生存和生产能力有很重要的意义。因此研究甘蓝型油菜中TFIIIA型锌指蛋白，对其进行挖据和鉴定有极其重要的价值。拟南芥属于十字花科，广泛用于植物遗传学、发育生物学和分子生物学的研究，是一种典型的模式植物。利用拟南芥中已命名的AT1G72050.2 TFIIIA型锌指蛋白为模板，采用生物信息学的方法，分析相关的理化信息和基因信息，通过同源序列的比对和进化树的构建，综合分析出甘蓝型油菜TFIIIA型锌指蛋白的进化历程。对以后的甘蓝型油菜TFIIIA型锌指蛋白的研究奠定基础。