摘要小鼠是哺乳动物研究实验中首选的一种模式生物。经过长时间的努力发展，培育转基因和基因打靶小鼠已经是很常见的事了，现在已经可以得到在核酸水平上的突变品系。目前，像这种基因组修饰变得越来越精确，而且在基因水平上标记不同的细胞群已变成非常普遍的研究[3]。89066

人类基因组计划（HGP）的完成之后，生物学研究中急需一种有效的基因功能分析方法。基因在疾病发生中的作用，从而揭示疾病的发生机制，推动医药学研究的发展。基因捕获和基因打靶是利用胚胎干细胞（ES细胞）生产基因敲除小鼠的两种不同技术。

目前Cre-LoxP重组酶系统在小鼠基因打靶中被广泛应用，相比传统的转基因技术，应用该系统的基因敲除具有时空特异性，优化了转基因小鼠模型。应用Cre-LoxP重组酶系统构建的转基因小鼠可在小鼠局部实现基因敲除，很大程度上避免小鼠过早死亡，为后续研究提供可行性。

本文通过基因敲入构建出Six3CreER转基因小鼠，并经过扩大繁殖。然后利用PCR技术鉴定筛选出成功构建的Six3Cre转基因小鼠，为后续的Cre-LoxP重组实验提供材料支持。

毕业论文关键词：Six3转基因小鼠；  PCR扩增； 生长发育； 基因型鉴定

Abstract Mouse is one of the preferred model organisms in mammalian research。 After a long period of hard work, it is a common practice to develop transgenic and gene targeting mice, which have now been able to obtain mutant lines at the nucleic acid level。 At present, genomic modifications such as this are becoming more and more precise, and the identification of different cell populations at the genetic level has become very common in the study of [3]。

After the completion of the human genome project (HGP), an effective method for gene function analysis is needed。 The role of genes in the pathogenesis of disease, thereby revealing the pathogenesis of disease and promoting the development of medical research。 Gene capture and gene targeting are two different techniques for producing knockout mice using embryonic stem cells (ES cells)。源Y于U优I尔O论P文W网wwW.yOueRw.com 原文+QQ75201-8766

At present, the Cre-LoxP recombinase system has been widely used in gene targeting in mice。 Compared with the traditional transgenic technology, the knockout of the system has the characteristics of spatial and temporal specificity, and the transgenic mouse model is optimized。 The transgenic mice constructed by Cre-LoxP recombinase system can realize gene knockout in mice, and avoid premature death to a large extent。

This paper constructed Six3CreER gene knock in transgenic mice, and after expanding reproduction。 Then the Six3Cre transgenic mice were successfully identified by PCR technology, which provided material support for the subsequent Cre-LoxP recombination experiments。

毕业论文关键词：Six3转基因小鼠；  PCR扩增； 生长发育； 基因型鉴定

Keyword: six3 transgenic mouse；PCR increase；grown and development;                  Genotype identification

目    录

第1章 引言 5

1。1 视网膜的意义 5

1。2 基因鉴定原理 5

1。3 Six3基因简介 6

1。4 视网膜疾病 6

第2章 实验试剂及仪器 6

2。1