摘 要：目的：本实验研究8%、15%浓度的酒精对小鼠记忆的影响。方法：将30只小白鼠分为三组，一组为对照组，饲养期间每天喂食蒸馏水，另外两组为实验组，一组每天喂食8%的酒精，一组每天喂食15%的酒精，饲养5w后，对三组小鼠分别进行水迷宫测试，记录其找到平台的时间。结果：与对照组相比，实验组小鼠的学习、记忆能力均表现出下降的趋势(p＜0。05 或p＜0。01)，15%浓度的小鼠记忆损伤比8%严重。结论：酒精对小鼠的记忆有明显的损害作用，导致学习记忆能力下降。78696

毕业论文关键词：小鼠，酒精，学习记忆，水迷宫

Abstract:Objective: the effects of 8% and 15% concentration of alcohol on the memory of mice were studied in this experiment。 Methods: 30 mice were randomly pided into three groups, a group among them was fed daily with distilled water daily as control, and the other two groups were treated as the experimental group by the means of feeding them 8% alcohol and 15% alcohol separately, three groups of mice were conducted with water maze test, also the time which the mice found the platform was recorded。 Results: compared with the control group, the the mice’s ability of learning and memory in the experimental group showed a trend of decrease (P < 0。05 or P < 0。01), and the 15% concentration of the memory impairment was more serious than 8%。 Conclusion: alcohol had significant effects on the memory of mice, leading to the decline of the ability of learning and memory。

Keywords:mice,alcohol,learning and memory,water maze test

目   录

1  前言 4

2  材料与方法 4

2。1  材料 4

2。1。1  实验材料、试剂及配制 4

2。1。2  实验仪器、用品 4

2。2  方法 4

2。2。1  动物分组、标记 4

2。2。2  动物喂养 4

2。2。3  通道式水迷宫 5

2。3  统计学处理 5

3  结果分析与讨论 5

3。1  通道式水迷宫实验结果 5

3。2  通道式水迷宫实验分析 6

3。3  讨论 7

3。3。1  学习和记忆的神经生理学机制 7

3。3。2  学习记忆的神经生物化学机制 8

3。3。3  学习记忆的神经干细胞假说 8

结论 10

参 考 文 献 11

致  谢 12

1  前言

    酒精中毒已经发展成全球普遍的常见病症，酒精依赖、酒精滥用已是世界各国较为常见的社会问题。酒精滥用与高犯罪、高离婚率、高交通事故率有明显相关。据有关报导[1],全球职业人口中大约有11%的人酗酒,给社会和家庭造成了非常大的经济损失;同时,对人类的健康也造成巨大威胁，尤其是在欧洲，其发病率仅次于心脑血管疾病和肿瘤；我国随着经济发展水平的不断提高，酒精中毒也逐渐增多，现在临床上对酒精中毒引起的神经系统损害现象日益增长。