木霉T-soybean诱导杨树抗盐性分析
毕业论文关键词：木霉T-soybean；杨树；诱导；抗盐性。
Salt Resistance Analysis of Poplar Induced by Trichoderma T-soybean
Abstract: Induction resistance of Trichoderma T-soybean on poplar was studied under salt stress. It was showed that compared to control plants, plants inoculated with Trichoderma T-soybean were much more flourishing, and size of leaves root length, plant height as well as number of lateral root increased significantly. Meanwhile, activities of POD, SOD, CAT, chlorophyll of content as well as root activity also increased significantly. Furthermore, Trichoderma T-soybean induced the resistance of poplar to salt. Compared to control, POD activity, SOD activity, CAT activity, chlorophyll content and root activity were improved. In sum, Trichoderma T-soybean induced resistance of poplar to salt.
Key words：Trichoderma T- soybean; Poplar; Induction; Salt resistance.
目 录
摘 要： 1
引言： 1
1.实验材料与方法 4
1.1实验所用仪器 4
1.2实验材料 4
1.3实验过程 4
1.3.1实验材料选择 4
1.3.2活化菌液 5
1.3.3植物处理 5
1.3.4木霉T-soybean对杨树生长的促进作用 5
1.3.5盐胁迫下，木霉T-soybean对杨树生长的影响 5
1.3.6盐胁迫下，木霉T-soybean对杨树生理指标的影响 5
2.结果分析 5
2.1生长指标 5
2.2生理指标测定 6
2.3.1盐胁迫条件下木霉处理对杨树叶片POD活性的影响 6
2.3.2盐胁迫条件下木霉处理对杨树叶片的SOD活性影响 7
2.3.3盐胁迫条件下木霉处理对杨树植株根系活力的影响 8
2.3.4盐胁迫条件下木霉处理对杨树植株CAT活性的影响 8
2.3.5盐胁迫条件下木霉处理对杨树植株叶绿素含量的影响 9
3.讨论 9
参考文献 10
致 谢 12
木霉T-soybean诱导杨树抗盐性分析
引言
木霉属真菌门，半知菌亚门，丝孢纲，丝孢目，丛梗孢科，是一种生物防治真菌，俗称环境友好型植病生物防治真菌。广泛存在于植物的根系周围环境中，即存在于不同环境条件下的土壤当中。木霉对植物种子的活力、株高、幼苗根系活性及幼苗叶片中叶绿素和可溶性蛋白含量等，都有着明显的增进作用，由此，科学家们从生化和分子水平上对拮抗木霉的生防机制进行一系列研究，并有较大的成功[1]。木霉可以使植物产生多种具有拮抗作用的生物活性物质，例如；细胞壁降解酶类和次级代谢产物，并能提高植物的抗逆性，促进植物生长以及提高农产品产量，因此被广泛应用于生物防治方面，或是生物肥料及土壤改良剂[2]。
木霉的生物防治机制：木霉菌防治病害或抑制病原菌的主要机制通常可以归为五种，即重寄生、产生抗生素、细胞壁分解酵素、营养竞争、以及诱导植物产生抗性。具体如下：拮抗作用、促生作用、诱导作用、竞争作用、重寄生作用。
下一篇：氰戊菊酯降解菌的选育及鉴定