茄科植物TIFY家族基因的鉴定与比较分析
毕业论文关键词：茄科植物；TIFY家族；基因；转录因子；进化
Molecular Identification and Comparative Analysis of the TIFY Family Genes in Solanaceae Species
Abstract: TIFY transcription factor superfamily in plants specific gene family plays an important role in the growth and development of plants in the process. At present, the model plant Arabidopsis to study TIFY family genes are more, but in the Solanaceae plant family genes related TIFY research are few. In this paper, tomato and potato as a representative of the Solanaceae, based on computational biology methods of these two species TIFY systematically analyze gene. Phylogenetic and sequence analysis revealed that 21 tomato and 21 potato TIFY genes were pided into four groups, each groups have similar gene structure and conserved motifs, but the structure and characteristics of the protein sequence of the gene between different groups There are obvious differentiation. For two pairs of paralogs tomato and potato selection pressure and expression profiling analysis showed: two pairs of paralogs are controlled by negative selection, with high conservation of adaptive evolution did not occur, but two pairs paralogs gene expression profile distinct differentiation occurs, indicating that the two pairs of homologous genes differentiation is mainly reflected in the gene expression profiles. These findings will help us to further study the biological function of the Solanaceae TIFY genes, but also for a deep understanding of the role TIFY protein in jasmonate signal transduction pathway theoretical basis.
Key words: Solanaceae, TIFY family, Gene, Transcription factor, Evolution
目 录
摘要 1
引言 2
1 数据与方法 3
1.1 基因检索与数据下载 3
1.2 基因结构分析 3
1.3 蛋白保守基序与结构域分析 3
1.4 序列联配与系统进化树构建 4
1.5 同源基因对的选择压力检测 4
1.6 同源基因对的表达分歧度分析 4
2 结果与分析 4
2.1 茄科植物TIFY家族基因的鉴定 4
2.2 茄科植物TIFY家族基因的结构特征 5
2.3 茄科植物TIFY蛋白的保守基序组成模式 7
2.4 茄科植物TIFY基因的系统进化关系 8
2.5 茄科植物TIFY同源基因的选择压力 10
2.6 茄科代表物种特异扩增同源基因对的表达情况 10
3 讨论与结论 11
参考文献 13
附录 14
致谢 15
茄科植物TIFY家族基因的鉴定与比较分析引言