不同土荆芥种群对锰铅胁迫的耐性生理研究
毕业论文关键词：土荆芥；种群；重金属；生理；耐性
The Physiological Mechanisms of Heavy Mental Tolerance in Chenopodium ambrosioides L. under Population Level
Abstract： Living in different concentrations of heavy mental for a long term, plants may produce a different tolerance or accumulation of ecological types. Chenopodium ambrosioides L. is an exotic invasive plant and have been in China for a long time. Its physiological mechanisms of heavy mental tolerance have not been reported thoroughly. Although there are studies claim that Chenopodium ambrosioides L. can hyper accumulate manganese and plumbum in solution culture condition and partially discussed its physiological activities under plumbum pollution, the exact physiological mechanisms of heavy mental tolerance are not elucidated and no research has been done under population level yet. This study, for the first time, take different populations of Chenopodium ambrosioides L. as material, trying to elucidate heavy mental tolerance mechanisms. In the meanwhile, this study may provide a feasible and practical new approach to repair polluted soil.
Key words: Chenopodium ambrosioides L.；Population；Heavy mental；Physiology; Tolerance
目 录
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）1
1材料与方法 2
1.1采样资料介绍 2
1.2土荆芥幼苗培养 3
1.3同质园培养 3
1.4土荆芥生理指标测定 3
1.4.1土荆芥形态指标测定 3
1.4.2土荆芥相对叶绿素含量测定 3
1.4.3土荆芥叶绿素荧光参数的测定 4
1.4.4土荆芥光合生理指标测定 4
1.4.5土荆芥MDA含量测定 4
1.4.6土荆芥可溶性蛋白含量测定 4
1.4.7土荆芥游离辅氨酸含量测定 4
1.4.8土荆芥叶、根细胞重金属含量 4
1.5数据处理 4
2结果与分析 4
2.1不同土荆芥种群对Mn的耐性及积累 4
2.1.1不同土荆芥种群Mn处理下形态指标测定 4
2.1.2不同土荆芥种群Mn处理下相对叶绿素含量 9
2.1.3不同土荆芥种群Mn处理下叶绿素荧光参数 10
2.1.4不同土荆芥种群Mn处理下光合生理指标 10
2.1.5不同土荆芥种群Mn处理下MDA含量 11
2.1.6不同土荆芥种群Mn处理下可溶性蛋白含量 11
2.1.7不同土荆芥种群Mn处理下游离辅氨酸含量 12
2.1.8不同土荆芥种群Mn处理下叶、根细胞重金属含量12
2.2不同土荆芥种群对Pb的耐性及积累 13
2.2.1不同土荆芥种群Pb处理下形态指标测定 13
2.2.2不同土荆芥种群Pb处理下相对叶绿素含量 18
2.2.3不同土荆芥种群Pb处理下叶绿素荧光参数 19
2.2.4不同土荆芥种群Pb处理下光合生理指标 19
2.2.5不同土荆芥种群Pb处理下MDA含量 20
2.2.6不同土荆芥种群Pb处理下可溶性蛋白含量 20
2.2.7不同土荆芥种群Pb处理下游离辅氨酸含量 21