亚精胺对黄瓜根抗旱性的影响
毕业论文关键词：干旱胁迫；亚精胺；丙二醛含量；CAT；POD；电导率；根系活力。
Effects of Spermidine on The Drought Resistance of Cucumber Roots
Abstract: Cucumber is greenhouse crops, meanwhile, it is also a new kind of green health food, The drought is a very important abiotic factors on its growth and development, therefore, adopt appropriate methods to improve the drought resistance of cucumber has very important significance in the agricultural production .PEG6000 simulated drought stress of two cucumber leaf stage ，By spraying spermidine as the experimental group ,with distilled water as the control group. Experiments indicate that, under drought stress, the activity of POD and CAT decreased more slowly than the untreated roots, but also inhibit extravasation drought electrolyte. The results indict that spermidine increased the tolerance of cucumber roots to drought. spermidine can enhanced the resistance of cucumber root to drought. The specific performance of it significantly enhanced activity of POD、CAT、the speed of the rise of MDA content change slowly and maintain higher root activity.
Keywords: Drought Stress; Spermidine; MDA Content; POD; CAT; Electrical Conductivity; Root Activity
目录
引言 1
1 材料与方法 2
1.1 材料 2
1.1.1供试材料 2
1.1.2黄瓜的培养和处理 2
1.1.3试剂 2
1.1.4设备 3
1.2测定项目与方法 3
1.2.1过氧化物酶（POD）活性测定（愈创木酚法） 3
1.2.2过氧化氢酶（CAT）活性测定（紫外吸收法） 4
1.2.3丙二醛（MDA）测定 5
1.2.4电导率的测定 5
1.2.5根系活力的测定 5
2结果与分析 6
2.1 亚精胺对干旱胁迫下的黄瓜根过氧化物酶（POD）活性的影响 6
2.2 亚精胺对干旱胁迫下的黄瓜根过氧化氢酶（CAT）活性的影响 7
2.3 亚精胺对干旱胁迫下黄瓜根丙二醛（MDA）含量的影响 7
2.4 亚精胺对干旱胁迫下黄瓜根电导率的影响 8
2.5 亚精胺对干旱胁迫下黄瓜根系活力的影响 9
3 讨论 9
4.参考文献 10
引言
植物常遭受的有害影响之一是缺水，干旱是世界最为严重的灾害之一。当植物所消耗的水分大于它吸收的水分时，就会导致组织内出现水分的亏缺，这种现象就称为干旱。干旱有两种：大气干旱和土壤干旱，大气干旱的主要特征是大气温度高而相对湿度低，造成蒸腾加强，所以水分平衡被破坏，我国的西北地区就常有大气干旱的出现。土壤干旱是指土壤中的水分缺乏，即植物的可吸收水分少，土壤干旱时，植物就会出现生长困难，所造成的作物受害情况大于大气干旱，我国的华北等地区常出现土壤干旱[1]。在干旱胁迫下多数植物的生物膜遭到破坏，导致细胞内的物质流向细胞外，因此造成膜系所文持的生理功能受到破坏[2-3]。据统计全世界除干旱之外的其他因素造成的作物减产的总和都不及水分胁迫所导致的作物减产[4]。