甘薯全基因组范围内转录本可变剪接的初步分析
毕业论文关键词：甘薯；可变剪接；功能注释；高通量测序；转录组
Genome-Wide Preliminary Analysis of Alternative Splicing in Sweet Potato
Abstract: Alternative splicing is common in higher eukaryotes and plays an important role in gene posttranscriptional regulation. Previously, we have performed high-throughput RNA sequencing in four samples, each of which originated from one sweetpotato accession. We got 218,091 Transcripts and 84,176 Unigenes after de novo assembly using Trinity. In total, 29.06% of the genes show features of alternative splicing and the majority of these genes have two alternative transcripts. These data indicate that alternative splicing may play an important role in the growth of sweet potato. Further, we classified their functional categories according the number of transcripts of each of these genes and found that the genes each having 2-5 transcripts were enriched in the two categories: (i) posttranslational modification, protein turnover, chaperones; (ii) translation, ribosomal structure and biogenesis. In addition, 10 genes were selected for further analysis of sequence alignments to show their putative patterns of alternative splicing. In general, our study provides data for understanding the transcriptomic mechanism in sweet potato.
Key words: Sweetpotato, alternative splicing, function annotation, high-throughput RNA sequencing, transcriptome
目录
第一章 引言 1
1.1 基因表达调控的机制类型 1
1.2 可变剪接 1
1.2.1 可变剪接的含义 1
1.2.2 可变剪接的分子机制 2
1.2.3 可变剪接的基本类型 3
1.2.4 可变剪接的生物学意义 4
1.3 基因组时代可变剪接研究的方法与进展 4
1.4甘薯物种及其研究现状简介 5
1.5本课题研究思路 5
第二章 材料与方法 6
2.1供试材料 6
2.2 基因功能注释的初步分析 6
2.3 可变剪接分析 8
第三章 结果与分析 10
3.1 功能注释分析结果 10
3.2 可变剪接分析结果 18
第四章 讨论 20
致谢 21
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