miRn-17在秦川牛不同时期不同组织的时空表达研究
毕业论文关键词：microRNA；病理调控；基因调控；肌肉生长
MiRn - 17 in space-time expression of qinchuan cattle groups at different time points
Abstract: Qinchuan cattle is one of the best in breed cattle, Qinchuan country has many excellent traits such as early maturity, marbling clear and the meat is tender and so on. However, there are still many shortcomings. In beef cattle rearing, muscle mass and growth is the most important measure of the performance of poultry production, research Qinchuan muscle growth factors are extremely important. In this study, in order to explore the miRn-17 gene in Qinchuan different growth stages, the expression of differences in the organization, we were collected from the fetal cattle, calves and cattle into different organs and different muscle as a sample experiments and quantitative real-time PCR and subjected to a series of data processing, we learned miRn-17 expression in fetal bovine muscle tissue relative to the same period in the spleen, lungs differential expression is very significant, and in the liver, kidney, back muscles The expression was not significantly different. In the calf, miRn-17 expression in muscle relative expression differences kidney, spleen is extremely significant, and heart, liver, fat no significant difference in the expression, the expression of differences in the lung significantly. Adult cattle, miRn-17 expression was prominent in the muscle, as opposed to other tissues is extremely significant difference. Qinchuan muscle at different times, the expression of test results miRn-17 is found in fetal calf muscle miRn-17 times the highest expression, calves and adult cattle expression and the expression of two relatively small amount of time or less. Overall miRn-17 expression in different tissues in different periods is having a good time and spatial expression differences.
Key words: MicroRNA; Pathological regulation; Gene regulation; Muscle growth
目录
引言 1
1实验材料及其试剂 2
1.1实验样本的采集 2
1.2 实验试剂 3
1.2.1 普通试剂 3
1.2.2 试剂盒类 3
1.3实验仪器 3
2实验方法步骤 4
2.1 引物设计 4
2.2 提取组织中的总RNA [9] 4
2.3 RNA的质量检测 5
2.4 RT-PCR 合成cDNA 5
2.4.1 除去总RNA中可能夹杂的基因组DNA 5
2.4.2 反转录反应 6