亚锰对鲫鱼肝脏SOD、GSH-PX及CAT的影响
毕业论文关键词：鲫鱼肝脏、锰、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）。
Effect of manganese on the activity of SOD、GSH-PX and CAT in Carassius auratus livers
Abstract: Manganese as a kind of necessary microelement for living beings, participates in many intracorporal metabolic activities. Antioxidase system assumes a leading role in scavenging free radicals and protecting animals organs, in which superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) are called protection enzyme system. The experiment arms to probe into how can manganese effect the fish's behaviors, weight and the activity of SOD, GPX and CAT. This experiment uses the Carassius auratus models exposed to 0.1 mol/L, 0.5 mol/L, 1 mol/L Mn2+ concentrations and contrasted with that from 0 mol/ L Mn2+. The experiment assaied behavior and activities of SOD, GPX and CAT in fish livers. The result shows that, after exposed to manganese, the fish weight lighter and behave decrease. The activity of SOD is obviously restrained, while the activity of GPX is inhibited as well. CAT activity had different change after exposed to manganese.
Keywords: Liver of Carassius auraust, Manganese, Superoxide dismutase (SOD), Glutathione peroxidase ( GSH-PX ), Catalase (CAT).
目录
引言 1
1 材料与方法 2
1.1 供试材料 2
1.1.1实验动物 2
1.1.2实验试剂 2
1.1.3实验设备 3
1.2 实验预处理 3
1.2.1样本预处理 4
1.2.2 样本采集 4
1.3指标的测定 4
1.3.1超氧化物歧化酶（SOD)的测定 4
1.3.2谷胱甘肽过氧化物酶(GSH-PX)的测定 4
1.3.3过氧化氢酶(CAT)的测定 5
2 结果与分析 6
2.1 鱼的行为 6
2.2 体重变化 7
2.3 肝超氧化物歧化酶活力 7
2.4 肝谷胱甘肽过氧化物酶活力 8
2.5 肝过氧化氢酶活力 9
3 讨论 10
4 致谢 11
参考文献 12
引言
由于现代工业的迅速发展，使得大量重金属流入江河湖泊，水域生态环境的平衡被打破，鱼类无法正常的生长和繁殖。而在食物链中，鱼类的位置是至关重要的，能够通过吸收水及沉淀物、摄食等方式在体内聚集重金属，达到相应的浓度之后，将会变成承载重金属的载体，增加了生态环境风险的同时也危害人类健康[1]。
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