紫苏醛对黑曲霉细胞周期和细胞凋亡的影响
毕业论文关键词：紫苏醛；黑曲霉；细胞周期；细胞凋亡
Effect of Perillaldehyde on Aspergillus niger cell cycle and apoptosis
Abstract:Perillaldehyde is one of the component extracted from perilla.It is considered to be one of the main active ingredient from the perilla essential oil antibacterial. This experiment by flow cytometry is based on that different concentrations of perillaldehyde have effect on A. niger cell cycle and cell apoptosis situation to study the antibacterial mechanism of perillaldehyde,which is mainly composed of three parts: the effect of perillaldehyde on sterol content of cell membrane of A. niger, the effect of perillaldehyde on the cell cycle of A. niger, the effect of perillaldehyde on the cells apoptosis of A. niger. Through the study of the three parts ,we found that perillaldehyde in different concentrations have different effects on A. niger under different time. The cell proportion of A. niger inhabited at G0/G1 phase rose from 26.8% to 33.6% ,29.8% to 42.5% under the treatment of 0.5 µL/mL,0.75 µL/mL perillaldehyde for 0 h,12 h,24 h or 48 h. The cell number of A. niger early apoptosis rose from 10.8% to 28.1%，17.5% to 41.1% under the treatment of 0.25 µL/mL,0.5 µL/mL perillaldehyde for 3 h,6 h or 9 h. From this experiment we also found that due to the influence of perillaldehyde, sterol content are also affected. The sterol content of cell membrane without perillaldehyde treatment of A. niger was about 0.50±0.13%, but under 0.125 µL/mL, 0.25 µL/mL, 0.5 µL/mL perillaldehyde treatment, the sterol content of A. niger were approximately 0.46±0.09%，0.32±0.03%，0.29±0.03%, so the function of perillaldehyde on A. niger is starting with the cell membrane of A. niger. It destructed the cell membrane of A. niger, then entered in the interior of the cell, in order to inhabit A. niger cell cycle and promote cell apoptosis, and inhibit the growth of A. niger and have the antibacterial effect. Through the preliminary study of perillaldehyde antibacterial mechanism, it got ready for deeper step research of perillaldehyde to provide certain theoretical basis. Perillaldehyde, as a natural preservative, is also healthier than synthetic preservatives, which has great potential development in terms of anticorrosive antibacterial.