黑曲霉Aspergillus nige对姜黄活性物质的微生物转化研究
毕业论文关键词：姜黄 姜黄素 黑曲霉 化学修饰
Niger Aspergillus on microbial transformation of active substances of turmeric
Abstract: The analysis of Curcuma longa L.as the traditional Chinese medicine resources value and research significance, Overview and progress of biological transformation is introduced. This experiment mainly through microbial transformation experiment of Aspergillus niger. Analysis of material changes and transformation activity before and after the comparison of turmeric, The results found: Curcuma longa L in the role of Aspergillus niger. The chemical composition was changed, determination by HPLC. The content of curcumin in Curcuma decreased from143.2μ g/ g to87.08μ g/ g. The results of LC- MS showed that the change of chemical ingredient in turmeric. The main component formed mostly through modified methylated and ethylated products. There are some alcohols or ketones by biological modification into alcohol or glycol. Confirmed by experiments of Aspergillus niger infection when the chemical constituents of Curcuma longa' s influence. To a certain extent affect the quality of turmeric turmeric and even change the drug. Through this experiment, the quality monitoring in turmeric storage in the process of analysis is provided theoretical basis and feasible method.
Key words：Curcuma longa L、 Curcumin、 Niger、Chemical modification
目录
前言： 5
1姜黄的研究进展与现状 5
1.1 姜黄的简介 5
1.2 姜黄的研究现状 5
2.生物转化的研究概况 6
2.1 生物转化的基本概念 6
2.2 生物转化的特点 6
2.2.1 专一性强 6
2.2.2副产物少、产量高 6
2.2.3反应条件温和： 7
2.2.4可以对化学方法难以进行的反应： 7
2.3 微生物转化的概况 7
2.3.1微生物转化概念： 7
2.3.2微生物转化优点:微生物转化的优点有很多： 7
2.3.4微生物转化的发展概况： 8
3.黑曲霉简介及研究进展 8
3.1黑曲霉简介 8
3.2黑曲霉的研究进展 8
材料与方法 9
2.1 菌种活化 9
2.2 接种 9
2.3 姜黄转化 9
2.4 实验组中姜黄素含量及转化物质分析 9
2.4.1色谱条件 9
2.4.2色-质谱分析 10
结果与讨论 10
3.1 色谱分析结果 10
3.3 姜黄转化后物质变化情况 12
四、结论 14
参考文献 14
引言：中国在使用中药的历史可谓历史悠久，最早的《神农百草经》就已经记载我国人民发现并使用中药。在五千年的文明发展过程中，许多中药典籍都为中国的中药发展奠定了坚实的基础。到了现代，许多药食两用的中药受到了大家的关注。姜黄是这其中较为常见的一种药食同源（药食两用）的中药。