光合细菌对小麦幼苗双酚A胁迫的缓解效应
毕业论文关键词：光合细菌；双酚A；生长；小麦；光合速率；抗氧化酶系
Effect of Photosynthetic Bacteria on Alleviating
Bisphenol A stress of Wheat Seedlings
Abstract: In order to explore the effects of photosynthetic bacteria on the growth of wheat seedlings in this paper, using the simulation lab experiment, the combined effects of 108 cfu/ml bacteria and varying concentrations of bisphenol A (BPA) (0, 50, 100, 200, 350, 500mg/kg) on physiological-biochemical characters of wheat seedlings were studied, in order to explore the influence of 108 cfu/ml photosynthetic bacteria on the seedlings growth and antioxidant enzyme activities in soybean under BPA stress. The results show that the photosynthetic rate, transpiration rate, stomatal conductance, the chlorophyll and soluble sugar content, peroxidase (POD) and catalase (CAT) activity of wheat seedlings reduced treated with the sole bisphenol A, whereas, malondialdehyde (MDA) content, Carbon dioxide absorption rate and the activity of Polyphenol oxidase (PPO). The low concentration of bisphenol A could increase the content of soluble sugar and the activities of the POD and the CAT. BPA with higher could decrease the photosynthetic rate, transpiration rate, stomatal conductance, the content of soluble sugar, the activities of POD and CAT. The 108cfu /ml alleviated the different concentrations of bisphenol A toxicity in wheat seedlings to various degree, but alleviative effect and BPA concentration had no significant. Compared to sole BPA treatment, the photosynthetic bacteria could increase the photosynthetic rate, transpiration rate, stomatal conductance, soluble sugar and antioxidant enzyme the activities of POD and CAT, whereas, the activities of PPO and MDA content of seedlings leaves decreased.
Key words: Photosynthetic bacteria; Bisphenol A; Growth; Wheat; Antioxidant Enzyme system.
目 录
摘 要 1
引言 2
1 材料与方法 3
1.1 试验材料 3
1.2 光合细菌的制备 3
1.3 试验设置与处理 3
1.4 仪器和设备 4
1.5 小麦生理指标的测定 4
1.6 数据处理 8
2 结果与分析 8
2.1 光合细菌与双酚A复合处理对小麦幼苗光合参数的影响 8
2.2 光合细菌与双酚A复合处理对小麦幼苗叶绿素含量的影响 9
2.3 光合细菌与双酚A复合处理对小麦幼苗可溶性糖含量的影响 10
2.4 光合细菌与双酚A复合处理对小麦幼苗PPO活性的影响 11