新型复合光催化剂对致病细菌的杀灭效应
关键词：沉淀-沉积法；BiOI/CNTs 光催化剂；金黄色葡萄球菌；抗菌性能
Killing effect of new type composite photocatalyst
on pathogenic bacteria
Abstract: BiOI/CNTs photocatalysts were prepared by hydrothermal method and precipitation – deposition method. The as-prepared samples were characterized with the techniques of X-ray diffraction (XRD), scanning electron microscope (SEM). The inactivation of Aureus Exterminate were performed with the as-prepared BiOI /CNTs and BiOI prepared by hydrothermal method and precipitation – deposition method to investigate their bactericidal property under visible light irradiation. The bactericidal activity of the as-prepared BiOI /CNTs and BiOI prepared by different methods were correlated with the chemical and physical property of the photocatalysts. The results showed different preparation method of BiOI /CNTs resulted in different bactericidal activity, BiOI/CNTs prepared by precipitation-deposition method had better antibacterial activity than the samples prepared by hydrothermal method and BiOI/CNTs had better antibacterial activity than BiOI.
Key Word： Precipitation–deposition method; BiOI photocatalyst；Staphylococcus aureus；Antibacterial property
摘要 1
引言 2
1 材料和方法 3
1.1实验材料 3
1.2实验材料的制备 3
1.2.1碳纳米管的预处理 3
1.2.2沉淀法制备BiOI/CNTs复合光催化剂 4
1.2.3 实验菌种 4
1.3 BiOI结构与性能表征 4
1.3.1 XRD分析 4
1.3.2 SEM分析 5
1.4 培养基的制备与灭菌 5
1.4.1培养基的制备 5
1.5 初始菌悬液的制备及浓度的测定 5
1.5.1菌种的活化 5
1.5.2初始菌悬液的制备 5
1.5.3平板法测初始菌悬液的浓度 5
1.6光催化剂的抗菌性能测定 6
2 结果与分析 6
2.1复合光催化剂的表征 6
2.1.1不同光催化剂的XRD分析 6
2.1.2沉淀法制备的BiOI/CNTs扫描电镜(SEM)图像 8
2.2复合光催化剂灭菌实验 9
3 讨论 10
参考文献 11
致 谢 12
新型复合光催化剂对致病细菌的杀灭效应 引言
伴随着科技的不断进步和工业的快速发展，水污染、大气污染、固体废弃物污染以及由此引发的细菌与病毒的肆意的繁殖和传播等，直接影响人类的生活质量、身体健康和生产活动。
抗菌剂和抗菌材料在缓解环境污染和杀灭细菌病毒方面做出了不可磨灭的贡献。但是，传统的无机抗菌剂、有机抗菌剂和天然生物系抗菌剂, 各有其优缺点[1]。例如：有机抗菌剂的抗菌效果好, 成本低廉, 但其耐久性差, 甚至毒性强；天然生物材料作为抗菌剂, 毒性和对环境污染小, 但受到安全性和加工条件的制约, 目前还不能实现大规模市场化生产；化学消毒剂和抗菌剂或多或少会有残留, 对环境产生污染, 甚至危害人体健康；利用某种微生物来达到消除或杀灭病原微生物的生物方法,过程缓慢, 效果不完全可靠, 且对细菌芽孢一般无杀灭作用。而半导体光催化剂，自发现以来，由于其具有表面活性大、热导性好、光吸收性好、分散性好等独特的性能引起了研究者的极大兴趣[2]。同时由于在实际应用中工艺流程简单、操作条件易控制、催化效率高、氧化能力强、无毒无害，无二次污染等优点, 使得半导体光催化剂在废水处理、大气净化、城市垃圾处理以及除臭杀菌等环保领域得到了广泛研究和应用[3]。