浮游植物叶绿素a测定方法的比较分析
毕业论文关键词：浮游植物；叶绿素a；抽滤；离心；单色法；三色法
Abstract
This study selected seven methods for the determination of chlorophyll a in Phytoplankton, including ultrasonic extraction spectrophotometric method hot ethanol, acetone heating, repeated freezing and thawing method, a thermal acetone, delay extraction and acetone heating - ultrasonic extraction spectrophotometry. Pond water sample was used to determine the content of chlorophyll a in Teaching Building D floor, Xiasha Campus, Hangzhou Normal University. The experiment compared effects of filtration and centrifugation operations on phytoplankton chlorophyll a measurement result, in accordance with extraction of chlorophyll a concentration, determination of the stability of the data and the simplicity of the experimental operation. The monochromatic method and the three color method for the determination of chlorophyll a were analyzed and discussed. Experimental results shown that, filtration and centrifugation operation on the determination results no significant influence, in view of the filtration operation time is short, simple operation, using vacuum filtration operation. Three color method for measuring the concentration of chlorophyll a is generally higher than the monochromatic method. On the whole, acetone heating method can be used as a common method for the measurement of phytoplankton chlorophyll a, because the method of measuring the chlorophyll a has high extraction efficiency, good data stability, and the operation is short and simple.
Key words: Phytoplankton; Chlorophyll a; Filtration; Centrifugal method; Monochromatic; Tristimulus method
目 录
0 引言 1
1 材料与方法 2
1.1 实验材料 2
1.2 主要仪器与设备 2
1.3 实验方法 2
1.3.1不同提取方法对浮游植物Chla含量测定的影响 2
1.3.2抽滤操作和离心操作对浮游植物Chla含量测定的影响 3
1.3.3单色法和三色法对浮游植物Chla含量测定的影响 3
1.3.4计算公式 3
1.4 数据分析 3
2 结果 4
2.1 7种方法对Chla提取效果的比较 4
2.2 抽滤操作和离心操作对浮游植物Chla含量测定结果比较 4
2.3 单色法、三色法测定Chla浓度的结果比较 4
2.4 不同提取方法实验耗时情况和变异系数分析 4
3 讨论 6
3.1 研究意义 6
3.2 7种方法对Chla提取效果的比较 7
3.3 抽滤操作和离心操作对浮游植物Chla含量测定结果比较 8